LY6G Antibody
LY6G, Rat Anti Mouse Antibody
Cat. No.
BT24327
Source
Synonyms
Lymphocyte antigen 6G, Ly-6G, Ly-6G.1, Ly6g, Gr1, Gr-1.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Product Specs
Introduction
Myeloid differentiation antigen Gr1 (Ly6G) is a GPI-anchored protein expressed briefly on monocytes in the bone marrow. Ly6G expression in the bone marrow directly correlates with granulocyte differentiation and maturation. The molecule's function was first identified when Ly6G ligation on murine neutrophils inhibited neutrophil recruitment. Primarily found on neutrophils, Ly6G is also present in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells.
Formulation
Supplied at a concentration of 1 mg/ml in PBS buffer (after reconstitution).
Shipping Conditions
The antibody is shipped in a lyophilized form at ambient temperature.
Storage Procedures
For long-term storage, keep the lyophilized antibody at 4°C in a dry environment. After reconstitution, aliquot and store at -20°C if not intended for use within one month.
Solubility
Reconstitute the antibody with distilled water (H2O). Gently mix the solution, ensuring to wash the sides of the vial, and allow it to sit for 30-60 seconds before use.
Applications
This antibody is suitable for flow cytometry, immunohistochemistry, and cell depletion applications. It specifically recognizes Ly-6G, expressed on most myeloid cells in the bone marrow and all peripheral granulocytes. For flow cytometry, the recommended concentration is 10 µl per 1 million cells (10^6 cells). The optimal titer for in vivo cell depletion (cytotoxicity) should be experimentally determined.
Available Conjugates
This antibody is also offered conjugated to biotin and FITC.
Synonyms
Lymphocyte antigen 6G, Ly-6G, Ly-6G.1, Ly6g, Gr1, Gr-1.
Purification Method
Protein A column.
Type
Rat Anti Mouse Monoclonal.
Clone
YRmLy-6G.
Immunogen
Ly-6G-transfected cell line.
Ig Subclass
Rat IgG2a.
Product Science Overview
Antibody Specificity and Clone Information
The Rat Anti-Mouse Ly-6G antibody, particularly the clone 1A8, is a monoclonal antibody that specifically recognizes the Ly-6G protein . This antibody is of the IgG2a, κ isotype and is produced by immunizing rats with Ly-6G transfected EL-4J cell lines . The clone 1A8 is known for its high specificity to Ly-6G and does not cross-react with Ly-6C, another member of the Ly-6 family .
Applications and Usage
The Rat Anti-Mouse Ly-6G antibody is widely used in various research applications, including:
- Flow Cytometry (FC): This antibody is quality control tested for immunofluorescent staining with flow cytometric analysis. It is recommended to use ≤ 0.25 µg per 10^6 cells in 100 µl volume .
- Immunohistochemistry (IHC): The antibody has been reported in the literature for use in both frozen and paraffin-embedded tissue sections .
- Neutrophil Depletion: Clone 1A8 has been used in vivo to deplete neutrophils in mice, which is useful for studying the role of neutrophils in various disease models .
Preparation and Storage
Technical Data and References
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