KRT17 Human

Cytokeratin 17 Human Recombinant
Cat. No.
BT27822
Source
Escherichia Coli.
Synonyms
Keratin 17, PCHC1, 39.1, CK-17, K17, PC2, PC, cytokeratin-17, Keratin 17 Epitope S1, Keratin 17 Epitope S2, Keratin 17 Epitope S4, Keratin, Type I Cytoskeletal 1, keratin-17, Cytokeratin-17, Keratin-17.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

KRT17 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 455 amino acids (1-432 a.a) and having a molecular mass of 50.5kDa.
KRT17 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Cytokeratin 17, also known as KRT17, is a type I cytokeratin found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. Mutations in the KRT17 gene can lead to disorders such as Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex. KRT17 plays a crucial role in the development and maintenance of various skin appendages, especially in determining hair shape and orientation. It also influences the function of TNF-alpha during hair cycling. Furthermore, KRT17 regulates protein synthesis and epithelial cell growth by binding to the adapter protein SFN and activating the Akt/mTOR pathway.
Description
Recombinant human KRT17, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein consists of 455 amino acids (with amino acids 1-432 derived from the KRT17 sequence) and has a molecular weight of 50.5 kDa. It includes a 23 amino acid His-tag at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The KRT17 protein solution is provided at a concentration of 0.5 mg/ml. The solution contains 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, and 0.4 M Urea.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the protein at -20°C. To ensure long-term stability, consider adding a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Purity
The purity of the KRT17 protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Keratin 17, PCHC1, 39.1, CK-17, K17, PC2, PC, cytokeratin-17, Keratin 17 Epitope S1, Keratin 17 Epitope S2, Keratin 17 Epitope S4, Keratin, Type I Cytoskeletal 1, keratin-17, Cytokeratin-17, Keratin-17.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMTTSIRQ FTSSSSIKGS SGLGGGSSRT SCRLSGGLGA GSCRLGSAGG LGSTLGGSSY SSCYSFGSGG GYGSSFGGVD GLLAGGEKAT MQNLNDRLAS YLDKVRALEE ANTELEVKIR DWYQRQAPGP ARDYSQYYRT IEELQNKILT ATVDNANILL QIDNARLAAD DFRTKFETEQ ALRLSVEADI NGLRRVLDEL TLARADLEMQ IENLKEELAY LKKNHEEEMN ALRGQVGGEI NVEMDAAPGV DLSRILNEMR DQYEKMAEKN RKDAEDWFFS KTEELNREVA TNSELVQSGK SEISELRRTM QALEIELQSQ LSMKASLEGN LAETENRYCV QLSQIQGLIG SVEEQLAQLR CEMEQQNQEY KILLDVKTRL EQEIATYRRL LEGEDAHLTQ YKKEPVTTRQ VRTIVEEVQD GKVISSREQV HQTTR

Product Science Overview

Introduction

Cytokeratin 17, also known as Keratin 17 or KRT17, is a type I intermediate filament protein that plays a crucial role in the structural integrity of epithelial cells. It is predominantly found in the nail beds, hair follicles, sebaceous glands, and other epidermal appendages . The recombinant form of this protein, referred to as Human Recombinant Cytokeratin 17, is produced using various expression systems for research and diagnostic purposes.

Structure and Expression

Cytokeratin 17 is composed of 432 amino acids and has a predicted molecular weight of approximately 73.26 kDa . The protein is expressed in wheat germ and is suitable for applications such as SDS-PAGE, ELISA, and Western Blotting . The sequence of Cytokeratin 17 includes several glycine-rich regions that contribute to its structural properties.

Biological Function

Cytokeratin 17 is involved in the formation and maintenance of various skin appendages. It plays a significant role in determining the shape and orientation of hair, as well as in the structural integrity of nail beds and sebaceous glands . The protein is also implicated in cellular processes such as proliferation, differentiation, and apoptosis.

Clinical Significance

Mutations in the KRT17 gene can lead to several genetic disorders, including Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex . These conditions are characterized by abnormalities in the skin, nails, and hair, highlighting the importance of Cytokeratin 17 in maintaining epithelial tissue integrity.

Applications in Research

Recombinant Human Cytokeratin 17 is widely used in research to study its role in epithelial biology and related diseases. It is utilized in various assays, including SDS-PAGE, ELISA, and Western Blotting, to investigate protein expression, function, and interactions . The recombinant protein is also valuable in the development of diagnostic tools and therapeutic strategies for skin-related disorders.

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