KRT17 Antibody

Cytokeratin 17, Mouse Anti Human
Cat. No.
BT22999
Source
Synonyms
Keratin 17, PCHC1, 39.1, CK-17, K17, PC2, PC, cytokeratin-17, Keratin 17 Epitope S1, Keratin 17 Epitope S2, Keratin 17 Epitope S4, Keratin, Type I Cytoskeletal 1, keratin-17, Cytokeratin-17, Keratin-17.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Cytokeratin 17, also known as KRT17, is a type I cytokeratin found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. Mutations in the KRT17 gene can lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex. KRT17 plays a role in forming and maintaining various skin appendages, particularly in determining hair shape and orientation. It also modulates TNF-alpha function in hair cycling. Additionally, KRT17 regulates protein synthesis and epithelial cell growth by binding to the adapter protein SFN and stimulating the Akt/mTOR pathway.
Physical Appearance
Clear, colorless liquid.
Formulation
Solution containing 1 mg/ml KRT17 antibody in PBS (pH 7.4), 10% glycerol, and 0.02% sodium azide.
Storage Procedures
Store at 4°C for up to 1 month. For longer-term storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
Stable for 12 months when stored at -20°C. Stable for 1 month when stored at 4°C.
Applications
This KRT17 antibody has been validated for ELISA and Western blot applications to ensure specificity and reactivity. However, optimal working dilutions should be determined by the researcher for each specific application. A starting dilution of 1:1000 is recommended.
Synonyms
Keratin 17, PCHC1, 39.1, CK-17, K17, PC2, PC, cytokeratin-17, Keratin 17 Epitope S1, Keratin 17 Epitope S2, Keratin 17 Epitope S4, Keratin, Type I Cytoskeletal 1, keratin-17, Cytokeratin-17, Keratin-17.
Purification Method
KRT17 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT9F3AT.
Immunogen
Anti-human KRT17 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human KRT17 protein 1-432 amino acids purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and k light chain.

Product Science Overview

Introduction

Cytokeratin 17 (CK17) is a type of intermediate filament protein that is part of the cytoskeleton in epithelial cells. It plays a crucial role in maintaining the structural integrity of cells and is involved in various cellular processes, including cell growth, differentiation, and apoptosis. CK17 is encoded by the KRT17 gene and is primarily expressed in the basal cells of complex epithelia.

Expression and Function

CK17 is typically found in the basal cells of complex epithelia but is not present in stratified or simple epithelia. It is expressed in various epithelial cells, including those in the bronchial epithelium and skin appendages. CK17 is considered a marker of basal cell differentiation and is often used to distinguish myoepithelial cells from luminal epithelium in glands such as mammary, sweat, and salivary glands .

Clinical Significance

CK17 expression is associated with several types of cancers, including small cell lung carcinoma (SCLC) and triple-negative breast carcinoma (TNBC). In TNBC, CK17 expression is linked to an aggressive clinical course. Additionally, CK17, along with other markers like MUC1, is used to define the pancreatobiliary subtype of cancers .

Mouse Anti-Human CK17 Antibody

Mouse anti-human CK17 antibodies are monoclonal antibodies developed to target and bind specifically to human CK17. These antibodies are used in various scientific applications, including immunohistochemistry (IHC), Western blotting, immunocytochemistry (ICC), flow cytometry, and enzyme-linked immunosorbent assay (ELISA). They are valuable tools in research and diagnostic settings for detecting CK17 expression in tissue samples .

Applications
  1. Immunohistochemistry (IHC): Used to visualize CK17 expression in formalin-fixed, paraffin-embedded tissue sections.
  2. Western Blotting: Employed to detect CK17 protein levels in cell lysates or tissue homogenates.
  3. Immunocytochemistry (ICC): Utilized to study CK17 expression in cultured cells.
  4. Flow Cytometry: Applied to analyze CK17 expression in cell populations.
  5. ELISA: Used for quantitative measurement of CK17 in biological samples .

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