KRT18 Human

Cytokeratin 18 Human Recombinant
Cat. No.
BT27908
Source
Escherichia Coli.
Synonyms
Keratin type I cytoskeletal 18, Cytokeratin-18, CK-18, Keratin-18, K18, KRT18,CYK18,Cell proliferation-inducing gene 46 protein.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by analysis by SDS-PAGE.
Usage

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Description

Cytokeratin 18 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain having a calculated molecular mass of 48,201 Dalton, showing a 45kDa band on SDS-page, pI-5.7.
The KRT18 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
The KRT18 gene provides instructions for making a protein called keratin 18. Keratin 18, along with its partner keratin 8, forms the internal structural framework of epithelial cells. Epithelial cells are found in the skin and the lining of internal organs. Specifically, keratin 8 and 18 are found in cells that form single-layered sheets of epithelium called simple epithelium. These keratins are the most abundant intermediate filament proteins in simple epithelial cells. Mutations in the KRT18 gene can cause a severe form of liver disease called cryptogenic cirrhosis.
Description
Recombinant Human Cytokeratin 18 is expressed in E. coli and purified. It is a single, non-glycosylated polypeptide chain with a calculated molecular weight of 48.2 kDa. However, it migrates with an apparent molecular weight of 45 kDa in SDS-PAGE, likely due to its high isoelectric point (pI) of 5.7.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The protein was lyophilized from a 1mg/ml solution containing 30mM Tris-HCl pH-8, 9.5M urea, 2mM EDTA, 2mM DTT and 10mM methylammonium chloride.
Solubility
Reconstitute the lyophilized CK-18 (1mg) in a minimum volume of 700 µl of sterile 18 MΩ-cm H₂O. Once fully dissolved, the solution can be further diluted in other aqueous buffers.
Stability
Lyophilized CK-18 can be stored at room temperature for up to 3 weeks, but it is recommended to store it at 2-8°C for optimal stability. After reconstitution, the protein should be stored at 4°C and used within 2-7 days. For long-term storage, it is advisable to add a carrier protein like HSA or BSA (0.1%) and store it below -18°C. Avoid repeated freeze-thaw cycles.
Purity
Greater than 95% as determined by SDS-PAGE analysis.
Reconstitution To Filaments
To form filaments, mix equal molar amounts of type I (e.g., CK-18) and type II cytokeratins, both dissolved in 9.5M urea buffer (see formulation) at a concentration of approximately 0.5 mg/ml. Protofilaments and filament complexes can then be obtained by stepwise dialysis of the polypeptide solution. First, dialyze against a 4M urea buffer, followed by dialysis against a low salt buffer (50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (e.g., Dulbecco's PBS).
Synonyms
Keratin type I cytoskeletal 18, Cytokeratin-18, CK-18, Keratin-18, K18, KRT18,CYK18,Cell proliferation-inducing gene 46 protein.
Source
Escherichia Coli.

Product Science Overview

Expression and Function

CK18 is predominantly expressed in single-layer epithelial tissues throughout the body, including the gastrointestinal tract, respiratory tract, urogenital tract, as well as endocrine and exocrine tissues . It plays a crucial role in maintaining the structural stability of these cells and is involved in various cellular processes such as cell signaling, apoptosis, and stress response .

Clinical Significance

Mutations in the CK18 gene have been linked to cryptogenic cirrhosis, a liver disease of unknown origin . Additionally, CK18 is a valuable biomarker in the diagnosis and prognosis of various cancers. It is present in a majority of adenocarcinomas and ductal carcinomas but is not typically found in squamous cell carcinomas . Hepatocellular carcinomas, a type of liver cancer, express only CK8 and CK18 .

Recombinant CK18

Recombinant human CK18 is produced using an expression system, typically in Escherichia coli (E. coli), and is purified to high levels of purity for research purposes . This recombinant protein is often tagged with a hexahistidine (His) tag to facilitate purification and detection in various assays . It is used as a positive control in immunological assays and is valuable in studying the protein’s function and interactions .

Applications in Research

CK18 is widely used in research to study epithelial cell biology, cancer, and liver diseases. Its expression patterns are utilized in distinguishing different types of epithelial malignancies through immunohistochemistry, cytopathology, and flow cytometric assays . The recombinant form of CK18 allows researchers to investigate its role in cellular processes and its interactions with other proteins under controlled conditions .

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