KRT14 Human, His
Description
KRT14 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 492 amino acids (1-472 a.a.) and having a molecular mass of 53.8kDa.
KRT14 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
MGSSHHHHHH SSGLVPRGSH MTTCSRQFTS SSSMKGSCGI GGGIGGGSSR ISSVLAGGSC RAPSTYGGGL SVSSSRFSSG GAYGLGGGYG GGFSSSSSSF GSGFGGGYGG GLGTGLGGGF GGGFAGGDGL LVGSEKVTMQ NLNDRLASYL DKVRALEEAN ADLEVKIRDW YQRQRPAEIK
DYSPYFKTIE DLRNKILTAT VDNANVLLQI DNARLAADDF RTKYETELNL RMSVEADING LRRVLDELTL ARADLEMQIE SLKEELAYLK KNHEEEMNAL RGQVGGDVNV EMDAAPGVDL SRILNEMRDQ YEKMAEKNRK DAEEWFFTKT EELNREVATN SELVQSGKSE ISELRRTMQN
LEIELQSQLS MKASLENSLE ETKGRYCMQL AQIQEMIGSV EEQLAQLRCE MEQQNQEYKI LLDVKTRLEQ EIATYRRLLE GEDAHLSSSQ FSSGSQSSRD VTSSSRQIRT KVMDVHDGKV VSTHEQVLRT KN.
Product Science Overview
Cytokeratin 14, also known as Keratin 14 or KRT14, is a type I intermediate filament protein that plays a crucial role in the structural integrity and function of epithelial cells. It is predominantly expressed in the basal cells of stratified epithelia, such as the epidermis, where it forms a network of filaments that provide mechanical support and resilience.
Cytokeratin 14 is composed of 472 amino acids and has a molecular weight of approximately 53.8 kDa . The protein is characterized by a central alpha-helical rod domain flanked by non-helical head and tail domains. The rod domain is responsible for the formation of coiled-coil dimers, which further assemble into intermediate filaments. These filaments interact with other cytoskeletal components to maintain cell shape, stability, and integrity.
The non-helical tail domain of Cytokeratin 14 is involved in promoting the self-organization of KRT5-KRT14 filaments into large bundles, enhancing the mechanical properties of keratin intermediate filaments . Additionally, a disulfide bond formed between filaments promotes the formation of a keratin filament cage around the nucleus, providing further structural support.
Recombinant Human Cytokeratin 14 (His Tag) is a laboratory-produced version of the native protein, expressed in Escherichia coli (E. coli) and purified using conventional chromatography techniques . The recombinant protein includes a His-tag at the N-terminus, which facilitates purification and detection. The His-tag is a short sequence of histidine residues that binds to nickel ions, allowing for easy isolation of the protein using nickel-affinity chromatography.
The recombinant Cytokeratin 14 protein is typically used in research applications, such as studying the protein’s structure, function, and interactions with other molecules. It is also employed in high-throughput screening assays and other experimental setups that require a reliable source of the protein .
Cytokeratin 14 is essential for the proper functioning of epithelial cells and plays a significant role in various biological processes, including cell growth, differentiation, and wound healing. Mutations in the KRT14 gene are associated with several genetic disorders, such as Epidermolysis Bullosa Simplex (EBS), a condition characterized by fragile skin that blisters easily .
Recombinant Human Cytokeratin 14 (His Tag) is a valuable tool for researchers studying these conditions and exploring potential therapeutic approaches. By providing a consistent and high-quality source of the protein, recombinant Cytokeratin 14 enables scientists to conduct detailed investigations into its role in health and disease.
