KRT20 Human

Cytokeratin 20 Human Recombinant
Cat. No.
BT28309
Source
Escherichia Coli.
Synonyms
Keratin type I cytoskeletal 20, Cytokeratin-20, CK-20, Keratin-20, K20, Protein IT, KRT20, CD20, CK20, KRT21, MGC35423.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Cytokeratin 20 Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain having a molecular mass of 48,553 Dalton.
The KRT20 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
KRT20, a member of the keratin family, is classified as a type I cytokeratin. Keratins, including cytokeratins and hair keratins, are intermediate filament proteins crucial for maintaining the structural integrity of epithelial cells. Type I cytokeratins are acidic proteins that pair with type II cytokeratins to form heterotypic keratin chains. KRT20 is predominantly found in mature enterocytes and goblet cells, showing specific expression in the gastric and intestinal mucosa. Notably, the genes encoding type I cytokeratins are clustered on chromosome 17q12-q21.
Description
Recombinant Human Cytokeratin 20, produced in E. coli, is a single, non-glycosylated polypeptide chain with a molecular weight of 48,553 Daltons. The purification of KRT20 is achieved through proprietary chromatographic methods.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein (1mg/ml) was lyophilized from a sterile solution containing 30mM Tris-HCL pH-8, 9.5M urea, 2mM DDT, 2mM EDTA, and 10mM methylammonium chloride.
Solubility
To reconstitute the lyophilized KRT20, it is recommended to dissolve it in sterile 18MΩ-cm H2O at a concentration of at least 100µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized KRT20 remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated below -18°C. After reconstitution, KRT20 should be stored at 4°C for 2-7 days. For future use, it should be stored below -18°C. To ensure stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is advisable. Avoid repeated freeze-thaw cycles.
Purity
The purity of KRT20 is determined to be greater than 95.0% by SDS-PAGE analysis.
Reconstitution To Filaments
To form filaments, mix equal molar amounts of type I and type II cytokeratins at a concentration of approximately 0.5 mg/ml. Both cytokeratins should be dissolved in a 9.5 M urea buffer (see above). Dialyze the polypeptide solution in a stepwise manner to a concentration of 4 M urea to obtain protofilaments and filament complexes. Subsequently, dialyze against a low salt buffer (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, further dialyze the solution against PBS (phosphate buffered saline, such as Dulbecco's PBS).
Synonyms
Keratin type I cytoskeletal 20, Cytokeratin-20, CK-20, Keratin-20, K20, Protein IT, KRT20, CD20, CK20, KRT21, MGC35423.
Source
Escherichia Coli.

Product Science Overview

Gene and Protein Structure

The gene encoding CK20 is located on chromosome 17q12-q21. The protein itself is a non-glycosylated polypeptide chain with a molecular mass of approximately 48,553 Daltons . The amino acid sequence of CK20 includes regions that are highly conserved among different species, with the highest antigen sequence identity to mouse and rat orthologs (77%) .

Expression and Function

CK20 is predominantly found in the epithelial cells of the gastrointestinal tract, including the stomach and intestines. It plays a crucial role in the structural organization and mechanical stability of these cells. The protein is involved in various cellular processes, including cell differentiation and proliferation .

Applications in Research

Recombinant CK20 is widely used in scientific research for various applications, including:

  • Immunohistochemistry (IHC): CK20 antibodies are used to detect the presence of CK20 in tissue samples, helping to identify specific cell types and diagnose certain diseases .
  • Western Blot (WB): CK20 is used as a marker in protein expression studies to analyze the presence and quantity of the protein in different samples .
  • Immunocytochemistry (ICC): CK20 is used to study the localization and distribution of the protein within cells .
  • Enzyme-Linked Immunosorbent Assay (ELISA): CK20 is used in assays to quantify the protein in various biological samples .
Recombinant CK20 Production

Recombinant CK20 is produced using various expression systems, including Escherichia coli (E. coli). The protein is purified using proprietary chromatographic techniques to ensure high purity and quality . Recombinant CK20 is available in different forms, including control fragments and full-length proteins, for use in blocking assays and other experimental applications .

Clinical Significance

CK20 is a valuable biomarker in clinical diagnostics. Its expression is often analyzed in various cancers, including colorectal cancer, gastric cancer, and bladder cancer. The presence of CK20 in tumor cells can provide important information about the origin and progression of the cancer, aiding in diagnosis and treatment planning .

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