KRT5 Human
Description
KRT5 is fused to a 23 amino acid His-tag at N-terminus.
Product Specs
Product Science Overview
Cytokeratins are a diverse group of proteins, with at least 20 different polypeptides identified in human epithelial tissues. Cytokeratin 5, specifically, is a type II keratin, which pairs with type I keratin (Cytokeratin 14) to form intermediate filaments. These filaments provide structural support and help cells withstand mechanical stress .
Cytokeratin 5 is predominantly expressed in the basal layer of stratified epithelia, such as the epidermis. It is also found in other epithelial tissues, including the respiratory tract and certain glandular tissues. The expression pattern of Cytokeratin 5 is often used as a marker to identify basal cells and to study epithelial cell differentiation and proliferation .
The expression of Cytokeratin 5 is significant in the context of various diseases, particularly cancers. It is used as a biomarker in the diagnosis and classification of epithelial malignancies. For instance, its presence can help distinguish between different types of carcinomas, such as basal cell carcinoma and squamous cell carcinoma .
Recombinant Cytokeratin 5 is produced using genetic engineering techniques, where the gene encoding Cytokeratin 5 is inserted into an expression system, such as E. coli, to produce the protein in large quantities. This recombinant protein is then purified and used in various research applications, including:
- Antibody Production: Recombinant Cytokeratin 5 is used as an antigen to generate specific antibodies for research and diagnostic purposes .
- Functional Studies: It is used in studies to understand the role of Cytokeratin 5 in cell structure, function, and disease mechanisms .
- Diagnostic Assays: Recombinant Cytokeratin 5 is employed in immunohistochemistry and other diagnostic assays to detect its expression in tissue samples .
The use of recombinant Cytokeratin 5 has facilitated numerous studies in cell biology and oncology. It helps researchers investigate the molecular mechanisms underlying epithelial cell behavior and the development of epithelial cancers. Additionally, it aids in the development of targeted therapies and diagnostic tools .
