KRT20 Human, His

Cytokeratin 20 Human Recombinant, His Tag
Cat. No.
BT28411
Source
Escherichia Coli.
Synonyms
Keratin, type I cytoskeletal 20, CD20, CK-20, CK20, K20, KRT21, Keratin, Cytokeratin-20, Keratin-20, Protein IT, KRT20.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

KRT20 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 447 amino acids (1-424 a.a) and having a molecular mass of 50.9kDa.
KRT20 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Recombinant Human Cytokeratin 20 His Tag (KRT20) is a member of the keratin protein family. Keratins are intermediate filament proteins that provide structural integrity to epithelial cells. They are categorized into cytokeratins and hair keratins. Type I cytokeratins consist of acidic proteins that form heterotypic keratin chain pairs. KRT20 is a major protein found in mature enterocytes and goblet cells, specifically expressed in the lining of the stomach and intestines.
Description
Recombinant Human KRT20, produced in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 447 amino acids (specifically, amino acids 1 to 424) and has a molecular weight of 50.9 kDa. This KRT20 protein is fused with a 23 amino acid His-tag at its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
The product is a sterile, colorless solution that has been filtered for sterility.
Formulation
The KRT20 protein is provided in a solution at a concentration of 0.5 mg/ml. The solution is buffered with 20mM Tris-HCl at a pH of 8.0 and also contains 0.4M Urea and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability during frozen storage, consider adding a carrier protein such as HSA or BSA to a final concentration of 0.1%. It is important to avoid repeated freezing and thawing of the product.
Purity
The purity of the KRT20 protein is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
Keratin, type I cytoskeletal 20, CD20, CK-20, CK20, K20, KRT21, Keratin, Cytokeratin-20, Keratin-20, Protein IT, KRT20.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMDFSRRS FHRSLSSSLQ APVVSTVGMQ RLGTTPSVYG GAGGRGIRIS NSRHTVNYGS DLTGGGDLFV GNEKMAMQNL NDRLASYLEK VRTLEQSNSK LEVQIKQWYE TNAPRAGRDY SAYYRQIEEL RSQIKDAQLQ NARCVLQIDN AKLAAEDFRL KYETERGIRL TVEADLQGLN KVFDDLTLHK TDLEIQIEEL NKDLALLKKE HQEEVDGLHK HLGNTVNVEV DAAPGLNLGV IMNEMRQKYE VMAQKNLQEA KEQFERQTAV LQQQVTVNTE ELKGTEVQLT ELRRTSQSLE IELQSHLSMK ESLEHTLEET KARYSSQLAN LQSLLSSLEA QLMQIRSNME RQNNEYHILL DIKTRLEQEI ATYRRLLEGE DVKTTEYQLS TLEERDIKKT RKIKTVVQEV VDGKVVSSEV KEVEENI.

Product Science Overview

Introduction

Cytokeratin 20 (CK20) is a type I cytokeratin, which is a member of the keratin family of proteins. These proteins are essential for the structural integrity of epithelial cells. Cytokeratins are subdivided into two types: type I (acidic) and type II (basic to neutral). CK20 is specifically expressed in the gastric and intestinal mucosa, making it a significant marker in the study of epithelial cell biology.

Structure and Expression

CK20 is a major cellular protein of mature enterocytes and goblet cells. The protein encoded by the CK20 gene consists of 424 amino acids and has a molecular weight of approximately 50.9 kDa . The recombinant form of CK20, fused with a His-tag at the N-terminus, is expressed in Escherichia coli (E. coli) and purified using chromatographic techniques .

Function and Significance

CK20 plays a crucial role in maintaining the structural integrity of epithelial cells. It forms intermediate filaments that provide mechanical support and stability to the cells. The expression of CK20 is highly specific to certain types of epithelial cells, making it a valuable marker in diagnostic pathology. It is often used to identify and differentiate between various types of carcinomas, particularly those originating in the gastrointestinal tract.

Recombinant CK20 with His Tag

The recombinant form of CK20, tagged with a His-tag, is widely used in research. The His-tag facilitates the purification and detection of the protein. The recombinant CK20 is produced in E. coli and is available in a purified form. It is typically stored in a buffer containing Tris-HCl, urea, and glycerol to maintain its stability .

Applications in Research

Recombinant CK20 is used in various research applications, including:

  • Cancer Research: CK20 is a valuable marker for studying gastrointestinal carcinomas and other epithelial cancers.
  • Cell Biology: It helps in understanding the structural and functional aspects of epithelial cells.
  • Diagnostic Pathology: CK20 is used as a diagnostic marker to differentiate between different types of epithelial tumors.

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