KRT16 Human

Cytokeratin 16 Human Recombinant
Cat. No.
BT27748
Source
Escherichia Coli.
Synonyms
Keratin, type I cytoskeletal 16, Cytokeratin 16, KRT16, Cytokeratin-16, CK-16, Keratin-16, K16, KRT16A, FNEPPK, K1CP, NEPPK.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

KRT16 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 496 amino acids (1-473) and having a molecular mass of 53.7 kDa.
KRT16 is fused to a 23 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Cytokeratin 16 (KRT16) belongs to the keratin family and plays a role in regulating innate immunity during skin barrier disruption. Essential for maintaining the skin barrier, KRT16 is co-expressed with keratin 14 in tissues like the esophagus, tongue, and hair follicles.
Description
This recombinant KRT16 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 496 amino acids (residues 1-473) with a molecular weight of 53.7 kDa. It includes an N-terminal 23 amino acid His-tag and undergoes purification via proprietary chromatographic methods.
Physical Appearance
A sterile, colorless solution.
Formulation
The KRT16 solution is provided at a concentration of 1 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.4 M Urea, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended periods, store frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Minimize repeated freeze-thaw cycles.
Purity
Purity exceeding 90.0% as determined by SDS-PAGE analysis.
Synonyms
Keratin, type I cytoskeletal 16, Cytokeratin 16, KRT16, Cytokeratin-16, CK-16, Keratin-16, K16, KRT16A, FNEPPK, K1CP, NEPPK.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMTTCSRQ FTSSSSMKGS CGIGGGIGGG SSRISSVLAG GSCRAPSTYG GGLSVSSRFS SGGACGLGGG YGGGFSSSSS FGSGFGGGYG GGLGAGFGGG LGAGFGGGFA GGDGLLVGSE KVTMQNLNDR LASYLDKVRA LEEANADLEV KIRDWYQRQR PSEIKDYSPY FKTIEDLRNK IIAATIENAQ PILQIDNARL AADDFRTKYE HELALRQTVE ADVNGLRRVL DELTLARTDL EMQIEGLKEE LAYLRKNHEE EMLALRGQTG GDVNVEMDAA PGVDLSRILN EMRDQYEQMA EKNRRDAETW FLSKTEELNK EVASNSELVQ SSRSEVTELR RVLQGLEIEL QSQLSMKASL ENSLEETKGR YCMQLSQIQG LIGSVEEQLA QLRCEMEQQS QEYQILLDVK TRLEQEIATY RRLLEGEDAH LSSQQASGQS YSSREVFTSS SSSSSRQTRP ILKEQSSSSF SQGQSS.

Product Science Overview

Structure and Expression

Cytokeratin 16 is encoded by the KRT16 gene and is typically expressed in the basal layer of stratified epithelial tissues, such as the skin, hair follicles, and nails. The protein consists of 473 amino acids and has a molecular weight of approximately 50 kDa . It forms heterodimers with type II keratins, such as KRT6A and KRT6B, to create a filamentous network within the cytoplasm of epithelial cells.

Function

The primary function of Cytokeratin 16 is to provide structural support to epithelial cells. It helps maintain the integrity of the skin and other epithelial tissues by forming a dense network of intermediate filaments. Additionally, Cytokeratin 16 plays a role in the regulation of cellular processes such as proliferation, differentiation, and apoptosis.

Clinical Significance

Mutations in the KRT16 gene are associated with several skin disorders, including:

  • Pachyonychia Congenita (PC): A rare genetic disorder characterized by thickened nails, painful calluses, and oral leukokeratosis.
  • Epidermolytic Palmoplantar Keratoderma (EPPK): A condition that causes thickening of the skin on the palms and soles.
Recombinant Cytokeratin 16

Recombinant Cytokeratin 16 is produced using various expression systems, such as Escherichia coli and wheat germ . The recombinant protein is often tagged with His or GST tags to facilitate purification and detection. It is used in research to study the protein’s structure, function, and interactions with other cellular components.

Applications

Recombinant Cytokeratin 16 is utilized in several applications, including:

  • SDS-PAGE: To analyze the protein’s purity and molecular weight.
  • Western Blotting: To detect the presence of Cytokeratin 16 in biological samples.
  • ELISA: To quantify the protein in various samples.
  • Affinity Purification: To isolate Cytokeratin 16 from complex mixtures.

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