KLK3, His

Kallikrein-3 Human Recombinant, His Tag
Cat. No.
BT4305
Source
Escherichia Coli.
Synonyms
Prostate-specific antigen, PSA, Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, Semenogelase, KLK3, APS, hK3, KLK2A1.
Appearance
Sterile Filtered clear solution.
Purity

Greater than 85.0% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

KLK3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 262 amino acids (25-261) and having a molecular mass of 28.8kDa.
The KLK3 is fused to a 25 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Kallikrein-3 (KLK3), a member of the kallikrein-related peptidase family, is a serine protease found in seminal plasma. KLK3 plays a crucial role in the liquefaction of the seminal coagulum by hydrolyzing semenogelin-1. This process is essential for normal fertility. Elevated serum levels of KLK3, also known as PSA in clinical settings, are a significant biomarker for prostate cancer diagnosis and monitoring. Beyond its reproductive role, KLK3 is implicated in carcinogenesis, making it a potential biomarker for other diseases.
Description
Recombinant human KLK3, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein consists of 262 amino acids (residues 25-261) with a molecular weight of 28.8 kDa. The KLK3 protein is purified using proprietary chromatographic techniques and includes a 25 amino acid His-Tag fused at the N-terminus.
Physical Appearance
Clear, sterile filtered solution.
Formulation
The KLK3 protein solution is provided at a concentration of 1mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0) and 0.4M Urea.
Stability
For short-term storage (up to 2-4 weeks), the KLK3 protein solution should be stored at 4°C. For extended storage, it is recommended to store the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. To maintain protein integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of KLK3 is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
Prostate-specific antigen, PSA, Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, Semenogelase, KLK3, APS, hK3, KLK2A1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMIVGGW ECEKHSQPWQ VLVASRGRAV CGGVLVHPQW VLTAAHCIRN KSVILLGRHS LFHPEDTGQV FQVSHSFPHP LYDMSLLKNR FLRPGDDSSH DLMLLRLSEP AELTDAVKVM DLPTQEPALG TTCYASGWGS IEPEEFLTPK KLQCVDLHVI SNDVCAQVHP QKVTKFMLCA GRWTGGKSTC SGDSGGPLVC NGVLQGITSW GSEPCALPER PSLYTKVVHY RKWIKDTIVA NP.

Product Science Overview

Structure and Expression

Kallikrein-3 is expressed from human 293 cells (HEK293) and carries a polyhistidine tag at the C-terminus. The protein has a calculated molecular weight of approximately 28.7 kDa, but it migrates as 33-36 kDa under reducing conditions due to glycosylation . The recombinant form of this protein is often used in research and clinical studies due to its high purity and activity .

Function

Kallikrein-3 plays a crucial role in the liquefaction of seminal coagulum by hydrolyzing semenogelin-1, which allows sperm to swim freely. This process is essential for normal male fertility. The enzyme’s catalytic activity is inhibited by SERPINA5 and strongly inhibited by zinc ions (Zn2+) .

Clinical Significance

Kallikrein-3 is widely known for its use as a biomarker in the diagnosis and monitoring of prostate cancer. Elevated levels of PSA in the blood can indicate the presence of prostate cancer, benign prostatic hyperplasia, or other prostate-related conditions. PSA is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT), in the sera of patients with prostatic carcinoma .

Recombinant Form

The recombinant form of Kallikrein-3, tagged with a polyhistidine (His) tag, is used extensively in research. This tag facilitates the purification and detection of the protein. The recombinant protein is lyophilized from a filtered solution and can be reconstituted for use in various assays. It is recommended to store the lyophilized product at -20°C or lower to maintain its stability .

Applications

Recombinant Kallikrein-3 is used in various research applications, including:

  • Biochemical studies: Understanding the enzyme’s structure, function, and inhibition.
  • Clinical research: Developing diagnostic assays for prostate cancer.
  • Drug development: Screening potential inhibitors of Kallikrein-3 for therapeutic purposes.

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