KLK3 Protein
Escherichia Coli.
Prostate-specific antigen, PSA, Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, Semenogelase, KLK3, APS, hK3, KLK2A1.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
Kallikrein-3 Human Recombinant produced in E.Coli is a single, non- glycosylated polypeptide chain containing 237 amino acids and having a molecular mass of 26.1kDa.
KLK3 is purified by proprietary chromatographic techniques.
Product Specs
Kallikrein-3 (KLK3), also known as Prostate Specific Antigen (PSA), belongs to the kallikrein-related peptidase family. Kallikreins are serine proteases with diverse physiological roles. Many kallikreins are involved in carcinogenesis, and some hold potential as biomarkers for cancer and other diseases. KLK3 is one of 15 kallikrein subfamily members located on chromosome 19. This protease, found in seminal plasma, plays a crucial role in the liquefaction of the seminal coagulum by breaking down semenogelin-1. In clinical settings, serum KLK3 levels, referred to as PSA levels, are valuable for diagnosing and monitoring prostate cancer.
Recombinant Human Kallikrein-3, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 237 amino acids. With a molecular weight of 26.1 kDa, KLK3 is purified using proprietary chromatographic techniques.
Sterile Filtered White lyophilized (freeze-dried) powder.
Lyophilized from a 0.2 μm filtered concentrated solution in 20mM Tris-HCl, pH 8.0, containing 150mM NaCl and 3% trehalose.
For reconstitution, dissolve the lyophilized Kallikrein-3 in sterile 18 MΩ-cm H₂O to a concentration of at least 100 μg/ml. This solution can be further diluted in other aqueous solutions as needed.
Lyophilized KLK3 remains stable at room temperature for up to 3 weeks. For long-term storage, keep desiccated at temperatures below -18°C. After reconstitution, store Kallikrein-3 at 4°C for 2-7 days. For future use, store below -18°C. Avoid repeated freeze-thaw cycles.
Purity exceeds 95.0% as determined by:
(a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis.
(b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
Prostate-specific antigen, PSA, Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, Semenogelase, KLK3, APS, hK3, KLK2A1.
Escherichia Coli.
IVGGWECEKH SQPWQVLVAS RGRAVCGGVL VHPQWVLTAA HCIRNKSVIL LGRHSLFHPE DTGQVFQVSH SFPHPLYDMS LLKNRFLRPG DDSSHDLMLL RLSEPAELTDA VKVMDLPTQE PALGTTCYAS GWGSIEPEEF LTPKKLQCVD LHVISNDVCA QVHPQKVTKF MLCAGRWTGG KSTCSGDSGG PLVCNGVLQG ITSWGSEPCA LPERPSLYTK VVHYRKWIKD TIVANP.
Product Science Overview
Kallikrein-3 is a glycoprotein with a molecular weight of approximately 30-35 kDa . It plays a crucial role in the male reproductive system by hydrolyzing semenogelin-1, a major protein in the seminal coagulum . This enzymatic activity leads to the liquefaction of the seminal clot, which is essential for sperm motility and fertility . Additionally, Kallikrein-3 is believed to help dissolve cervical mucus, facilitating sperm entry into the female reproductive tract .
Kallikrein-3 is widely known for its use as a biomarker in the diagnosis and monitoring of prostate cancer . Elevated levels of PSA in the blood can indicate the presence of prostate cancer, benign prostatic hyperplasia, or prostatitis. The measurement of PSA levels is a common screening tool used to detect prostate abnormalities at an early stage .
Recombinant human Kallikrein-3 is produced using various expression systems, including bacterial, yeast, and mammalian cells . The recombinant protein is often tagged with a histidine tag to facilitate purification and is typically lyophilized for storage . The recombinant form retains the enzymatic activity of the native protein and is used in various research applications, including the study of prostate cancer and the development of diagnostic assays .
Recombinant Kallikrein-3 is utilized in numerous research areas, such as:
- Cancer Research: Studying the role of PSA in prostate cancer progression and metastasis.
- Diagnostic Development: Developing and validating assays for PSA detection in clinical samples.
- Protein Engineering: Investigating the structure-function relationship of Kallikrein-3 and engineering variants with altered enzymatic properties.
