KLK3 Human, HEK
HEK293 Cells.
Prostate-specific antigen, PSA, Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, Semenogelase, KLK3, APS, hK3, KLK2A1
Greater than 95.0% as determined by SDS-PAGE.
Description
KLK3 Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (18-261 a.a) containing a total of 250 amino acids, having a molecular mass of 27.6kDa.
KLK3 is fused to a 6 amino acid His-tag at C-terminus,and is purified by proprietary chromatographic techniques.
Product Specs
Kallikrein-3 (KLK3), a serine protease, plays a crucial role in the breakdown of proteins. Specifically found in the prostate gland, KLK3 is involved in the liquefaction of semen by breaking down seminogelin. In men with healthy prostates, KLK3 levels are typically low. Elevated levels of KLK3 in serum can indicate various prostate conditions, including prostate cancer, prostatitis (inflammation of the prostate), and benign prostatic hyperplasia (enlarged prostate).
Recombinant human KLK3, produced in HEK cells, is a single-chain polypeptide. It undergoes glycosylation and consists of 250 amino acids (18-261 a.a), resulting in a molecular weight of 27.6 kDa.
For purification purposes, a 6-amino acid His-tag is fused to the C-terminus of KLK3, and proprietary chromatographic techniques are employed.
The KLK3 solution is prepared at a concentration of 0.25 mg/ml. It is formulated in a solution containing 10% glycerol and Phosphate-Buffered Saline (pH 7.4).
Repeated freezing and thawing of the KLK3 solution should be avoided.
The purity of KLK3 is determined to be greater than 95.0% using SDS-PAGE analysis.
KLK3 exhibits a specific activity greater than 250 pmol/min/ug. This activity is determined by measuring the amount of enzyme required to cleave 1 picomole of the substrate Succinyl-ArgPro-Tyr-p-Nitroanilide per minute at a pH of 7.5 and a temperature of 37°C. This cleavage results in the formation of Succinyl-Arg-Pro-Tyr and p-Nitroanilide.
Prostate-specific antigen, PSA, Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, Semenogelase, KLK3, APS, hK3, KLK2A1
HEK293 Cells.
APLILSRIVG GWECEKHSQP WQVLVASRGR AVCGGVLVHP QWVLTAAHCI RNKSVILLGR HSLFHPEDTG QVFQVSHSFP HPLYDMSLLK NRFLRPGDDS SHDLMLLRLS EPAELTDAVK VMDLPTQEPA LGTTCYASGW GSIEPEEFLT PKKLQCVDLH VISNDVCAQV HPQKVTKFML CAGRWTGGKS TCSGDSGGPL VCNGVLQGIT SWGSEPCALP ERPSLYTKVV HYRKWIKDTI VANPHHHHHH
Product Science Overview
Kallikrein-3 (KLK3), also known as plasma kallikrein (PKK) or prostate-specific antigen (PSA), is a serine protease that plays a crucial role in various physiological processes. It is widely studied for its involvement in blood pressure regulation, complement activation, and inflammatory response control .
KLK3 was first identified in relation to the Fletcher trait, a hereditary disorder characterized by an extended partial thromboplastin time, which normalizes upon exposure to a clot-promoting surface . The gene encoding KLK3 is located on human chromosome 4q35 and consists of a leader peptide of 19 residues and a 619-residue mature prekallikrein protein .
The mature KLK3 protein contains a 371-residue N-terminal heavy chain composed of four Apple domains, homologous to those found in factor XI . Both the heavy and light chains of KLK3 are highly glycosylated, with the light chain existing in 36- and 33-kDa isoforms due to variations in the carbohydrate moiety . The recombinant form of KLK3 is expressed in HEK 293 cells and is tagged with a 6-His tag at the C-terminus .
KLK3 is a key component of the kinin-generating pathways and the surface-mediated "contact system" . It is involved in:
- Blood Pressure Modulation: KLK3 helps regulate blood pressure by generating kinins, which are vasodilatory peptides.
- Complement Activation: It plays a role in the activation of the complement system, which is part of the immune response.
- Inflammatory Response: KLK3 is crucial in controlling and maintaining inflammatory responses .
