KLK3 Human, Native
Human seminal fluid.
Prostate-specific antigen, PSA, Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, Semenogelase, KLK3, APS, hK3, KLK2A1
Sterile Filtered clear solution.
Greater than 96.0%.
Description
Human Kallikrein-3 produced in Human seminal fluid having a molecular mass of approximately 30kD.
Product Specs
Kallikrein-3 (KLK3), a member of the kallikrein-related peptidase family, is a serine protease found in seminal plasma. Kallikreins play diverse roles in physiological processes, and some are implicated in carcinogenesis and serve as potential biomarkers for cancer and other diseases. KLK3 is one of 15 kallikrein subfamily members clustered on chromosome 19. It plays a crucial role in the liquefaction of the seminal coagulum by hydrolyzing semenogelin-1. This enzymatic activity is essential for normal seminal fluid liquefaction. Clinically known as PSA, KLK3 protein levels in serum are valuable for diagnosing and monitoring prostate cancer.
Native human Kallikrein-3, isolated from human seminal fluid, with an approximate molecular weight of 30 kDa.
A clear, sterile-filtered solution.
The protein is supplied in a solution of 0.09% NaN3, 0.05M phosphate buffer, 150mM NaCl, pH 7.5, and has been filtered through a 0.2 μm filter.
Store Human Kallikrein-3 at a temperature between 2°C and 8°C. Freezing is not recommended.
The purity is determined to be greater than 96.0%.
The donor of the starting material has undergone testing and received certification for negative antibody results for HIV-1, HIV-2, HCV, HBSAG, and Syphilis. Additionally, the donor has tested negative for HIV/HBV/HCV by PCR.
Prostate-specific antigen, PSA, Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, Semenogelase, KLK3, APS, hK3, KLK2A1
Human seminal fluid.
Product Science Overview
Kallikrein-3 is a glycoprotein enzyme with a molecular mass of approximately 28 kDa, although it appears as a 36 kDa protein under reducing conditions in SDS-PAGE . The enzyme is initially synthesized as a proenzyme and undergoes post-translational modifications to become active . The active form of Kallikrein-3 is involved in the cleavage of various peptide substrates, which is crucial for its biological functions .
The primary biological role of Kallikrein-3 is in the liquefaction of semen. It cleaves semenogelin, a major protein in the seminal coagulum, leading to the liquefaction of the seminal fluid . This process is essential for sperm motility and fertility. Additionally, Kallikrein-3 has been implicated in various physiological and pathological processes, including tissue remodeling and cancer progression .
Kallikrein-3 is best known for its clinical significance as a biomarker for prostate cancer. Elevated levels of PSA in the blood can indicate the presence of prostate cancer, benign prostatic hyperplasia, or prostatitis . PSA testing is widely used for the early detection and monitoring of prostate cancer, although it is not without limitations. Elevated PSA levels can also be seen in non-cancerous conditions, leading to potential false positives .
Research on Kallikrein-3 has expanded beyond its role as a biomarker. Studies have explored its potential as a therapeutic target for prostate cancer and other diseases . Recombinant forms of Kallikrein-3 are used in various research applications, including the study of enzyme kinetics, substrate specificity, and inhibitor screening .
