KLK3 Antibody

Kallikrein-3, Mouse Anti Human
Cat. No.
BT20519
Source
Synonyms
Prostate-specific antigen, PSA, Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, Semenogelase, KLK3, APS, hK3, KLK2A1.
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Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Kallikrein-3 (KLK3), a member of the kallikrein-related peptidase family, plays a crucial role in the physiological functions of the human body. Kallikreins, a subgroup of serine proteases, exhibit diverse functions, with many implicated in carcinogenesis. This makes some kallikreins potential biomarkers for cancer and other diseases. KLK3, one of the 15 kallikrein subfamily members clustered on chromosome 19, is primarily found in seminal plasma. Its primary function is to break down semenogelin-1, a process that leads to the liquefaction of the seminal coagulum. This liquefaction is crucial for normal sperm motility. Clinically, KLK3 is known as PSA (Prostate-Specific Antigen). Measuring serum PSA levels is an important diagnostic and monitoring tool for prostate cancer.
Formulation
The antibody is supplied as a 1mg/ml solution in a buffer consisting of PBS at a pH of 7.4, with 0.1% Sodium Azide added as a preservative.
Storage Procedures
For short-term storage of up to one month, the antibody should be kept at a refrigerated temperature of 4°C. For long-term storage, it is recommended to store the antibody at -20°C. Repeated freezing and thawing of the antibody should be avoided to maintain its stability and activity.
Stability / Shelf Life
The antibody remains stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
The KLK3 antibody has undergone rigorous testing using ELISA and Western blot analysis to confirm its specificity and reactivity. However, as specific experimental conditions may vary, it is recommended to optimize the antibody concentration for each application to achieve the best results. For Western blot analysis, a dilution range of 1:500 to 1:5000 is suggested as a starting point.
Synonyms
Prostate-specific antigen, PSA, Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, Semenogelase, KLK3, APS, hK3, KLK2A1.
Purification Method
KLK3 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
P1D10AT.
Immunogen
Anti-human KLK3 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human KLK3 amino acids 25-261 purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and ? light chain.

Product Science Overview

Introduction

Kallikrein-3, also known as prostate-specific antigen (PSA), is a serine protease enzyme encoded by the KLK3 gene. It is a member of the human tissue kallikrein family, which consists of 15 serine proteases. PSA is predominantly produced by the epithelial cells of the prostate gland and is secreted into the seminal plasma in high concentrations. It plays a crucial role in the liquefaction of semen by cleaving semenogelins, which are major gel-forming proteins in the seminal fluid.

Structure and Function

Kallikrein-3 is a glycoprotein with a molecular weight of approximately 28-34 kDa. It is synthesized as an inactive zymogen and undergoes proteolytic cleavage to become an active enzyme. The active form of PSA exhibits chymotrypsin-like activity, which is essential for its role in semen liquefaction. PSA is also involved in the degradation of extracellular matrix proteins, which may facilitate the invasion and metastasis of prostate cancer cells.

Clinical Significance

PSA is widely used as a biomarker for the diagnosis and monitoring of prostate cancer. Elevated levels of PSA in the blood can indicate the presence of prostate cancer, benign prostatic hyperplasia (BPH), or prostatitis. PSA testing is commonly used in conjunction with other diagnostic tools, such as digital rectal examination (DRE) and prostate biopsy, to improve the accuracy of prostate cancer diagnosis.

Mouse Anti-Human Kallikrein-3 Antibodies

Mouse anti-human kallikrein-3 antibodies are monoclonal antibodies generated by immunizing mice with human PSA. These antibodies are highly specific to human PSA and are used in various research and diagnostic applications. They can be used in techniques such as Western blotting, immunohistochemistry, immunoprecipitation, and enzyme-linked immunosorbent assay (ELISA) to detect and quantify PSA levels in biological samples.

Applications in Research

Mouse anti-human kallikrein-3 antibodies are valuable tools in prostate cancer research. They are used to study the expression and regulation of PSA in prostate cancer cells and tissues. These antibodies can also be employed to investigate the molecular mechanisms underlying PSA’s role in prostate cancer progression and metastasis. Additionally, they are used in the development of PSA-based diagnostic assays and therapeutic strategies.

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