IP 10 Rhesus macaque
C-X-C motif chemokine 10, 10 kDa, Gamma-IP10, IP-10, Small-inducible cytokine B10, CXCL10, SCYB10.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
The IP-10 is purified by proprietary chromatographic techniques.
Product Specs
Chemokine (C-X-C motif) ligand 10 (CXCL10), also called 10 kDa IP-10, is a small cytokine of the CXC chemokine family. Secreted by monocytes, endothelial cells, fibroblasts, and other cell types in response to IFN-γ, CXCL10 plays various roles, including attracting monocytes and T cells, facilitating T cell adhesion to endothelial cells, inhibiting tumor growth and bone marrow colony formation, and suppressing angiogenesis. The gene encoding CXCL10 is found on human chromosome 4 within a cluster of several other CXC chemokines. This chemokine exerts its effects by binding to the CXCR3 receptor on the cell surface. Its three-dimensional structure has been elucidated under three different conditions to a resolution of 1.92A.
(a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis.
(b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
C-X-C motif chemokine 10, 10 kDa, Gamma-IP10, IP-10, Small-inducible cytokine B10, CXCL10, SCYB10.
Product Science Overview
IP-10, also known as C-X-C motif chemokine 10 (CXCL10), is a small cytokine belonging to the CXC chemokine family. It is also referred to as 10 kDa interferon gamma-induced protein (IP-10). This chemokine is secreted by various cell types, including monocytes, endothelial cells, and fibroblasts, in response to interferon-gamma (IFN-γ) .
CXCL10 plays several critical roles in the immune system:
- Chemoattraction: It attracts monocytes, T cells, and other immune cells to sites of inflammation.
- T Cell Adhesion: Promotes the adhesion of T cells to endothelial cells, facilitating their migration to inflamed tissues.
- Antitumor Activity: Exhibits antitumor properties by inhibiting tumor growth and angiogenesis.
- Inhibition of Bone Marrow Colony Formation: Suppresses the formation of bone marrow colonies, impacting hematopoiesis .
The gene encoding CXCL10 is located on human chromosome 4, within a cluster of other CXC chemokines. The protein itself is a non-glycosylated polypeptide chain consisting of 77 amino acids, with a molecular mass of approximately 8.7 kDa . The three-dimensional crystal structure of CXCL10 has been determined under various conditions, providing insights into its functional mechanisms .
The recombinant form of IP-10 from Rhesus macaque is produced in Escherichia coli (E. coli). This recombinant protein is a single, non-glycosylated polypeptide chain containing 77 amino acids. It is purified using proprietary chromatographic techniques to ensure high purity and biological activity .
- Appearance: Sterile filtered white lyophilized (freeze-dried) powder.
- Formulation: Lyophilized from a 0.2 µm filtered concentrated solution in 1×PBS, pH 7.4.
- Solubility: Recommended to reconstitute in sterile water to a concentration of at least 100 µg/ml, which can then be further diluted.
- Stability: Stable at room temperature for up to 3 weeks when lyophilized. Upon reconstitution, it should be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term storage. Adding a carrier protein like HSA or BSA is recommended to prevent freeze-thaw cycles .
