Introduction
Chemokine (C-X-C motif) ligand 10 (CXCL10), also known as IP-10, is a small cytokine that belongs to the CXC chemokine family. It is produced and secreted by various cell types, including monocytes, endothelial cells, and fibroblasts, in response to inflammatory stimuli. CXCL10 plays a crucial role in immune responses by attracting immune cells, such as monocytes and T cells, to the site of inflammation. It exerts its effects by binding to the CXCR3 receptor on the surface of these cells. CXCL10 has been implicated in a variety of physiological processes, including chemotaxis, T cell adhesion, tumor suppression, and inhibition of angiogenesis. Structurally, CXCL10 is a protein with a three-dimensional structure that has been extensively studied. The gene encoding CXCL10 is found on human chromosome 4 in close proximity to other CXC chemokine genes.
Description
This product is a recombinant IP-10 protein produced in E. coli. It is a single, non-glycosylated polypeptide chain consisting of 77 amino acids, with a molecular weight of 8.7 kDa. The protein is purified using proprietary chromatographic techniques, ensuring high purity.
Physical Appearance
Sterile Filtered White lyophilized powder
Formulation
IP-10 was lyophilized from a 0.2µm filtered concentrated solution in 1xPBS, pH 7.4.
Solubility
To reconstitute the lyophilized IP-10, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. This solution can then be further diluted into other aqueous solutions as needed.
Stability
Lyophilized IP-10 is stable at room temperature for up to 3 weeks; however, it is recommended to store it desiccated at -18°C for long-term storage. After reconstitution, CXCL10 can be stored at 4°C for 2-7 days. For prolonged storage, adding a carrier protein like HSA or BSA (0.1%) is recommended. Avoid repeated freeze-thaw cycles.
Purity
Greater than 97% as determined by: (a) RP-HPLC analysis (b) SDS-PAGE analysis
Biological Activity
The biological activity of this product is determined based on its ability to chemoattract hCXCR3/HEK293 cells. The chemoattractant activity is measured using a concentration range of 100-500 ng/ml.
Protein Content
Protein quantitation was performed using two independent methods: 1. UV spectroscopy at 280 nm using an extinction coefficient of 0.02 for a 0.1% (1 mg/ml) solution. This value was calculated using the PC GENE computer analysis program for protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IP-10 as a Reference Standard.
Synonyms
Small inducible cytokine B10, CXCL10, 10 kDa, Gamma-IP10, IP-10, chemokine (C-X-C motif) ligand 10, C7, IFI10, INP10, crg-2, mob-1, SCYB10, gIP-10.
Amino Acid Sequence
IPLARTVRCN CIHIDDGPVR MRAIGKLEII PASLSCPRVE IIATMKKNDE QRCLNPESKT IKNLMKAFSQ KRSKRAP.
