IP 10 Rat

The gene encoding CXCL10 is located on human chromosome 4 and is part of a cluster of CXC chemokines . The rat recombinant version of CXCL10 is a single non-glycosylated polypeptide chain containing 77 amino acids . The three-dimensional crystal structure of CXCL10 has been determined under different conditions, providing insights into its functional mechanisms .

CXCL10 is secreted by several cell types, including monocytes, endothelial cells, and fibroblasts, in response to interferon-gamma (IFN-γ) . It has multiple roles in the immune system, such as:

• Chemoattraction: Attracts monocytes, macrophages, T cells, NK cells, and dendritic cells to sites of inflammation .
• Promotion of T cell adhesion: Enhances the adhesion of T cells to endothelial cells .
• Antitumor activity: Exhibits properties that inhibit tumor growth .
• Inhibition of angiogenesis: Prevents the formation of new blood vessels .

CXCL10 has been identified as a biomarker for various diseases. Elevated levels of CXCL10 are associated with chronic infections, such as hepatitis C virus (HCV) infection, and can predict the response to antiviral therapy . Additionally, CXCL10 plays a role in liver disease, where it recruits activated T cells to the liver parenchyma, contributing to disease progression .

Recombinant rat CXCL10 is produced using E. coli expression systems and is purified to high standards to ensure biological activity . It is used in various research applications, including chemotaxis bioassays and studies on immune responses . The recombinant protein is fully biologically active and has been validated on appropriate cell lines .

Recombinant rat CXCL10 is typically lyophilized and should be stored at -20°C to -70°C for long-term storage . Upon reconstitution, it is stable at 2-8°C for short-term use and should be aliquoted to avoid repeated freeze-thaw cycles .