IP 10 Human, His

IP-10 is an 8.7 kDa protein that is secreted by several cell types in response to interferon-gamma (IFN-γ). The recombinant form of IP-10, often tagged with a histidine (His) tag for purification purposes, is produced in various expression systems, including Escherichia coli . The His tag facilitates the purification of the protein using nickel affinity chromatography, ensuring a high degree of purity .

IP-10 plays a crucial role in the immune response. It acts as a chemoattractant for monocytes/macrophages, T cells, natural killer (NK) cells, and dendritic cells . Additionally, it promotes T cell adhesion to endothelial cells, exhibits antitumor activity, and inhibits bone marrow colony formation and angiogenesis .

IP-10 exerts its effects by binding to the C-X-C motif chemokine receptor 3 (CXCR3), a seven-transmembrane receptor coupled to G proteins . This interaction triggers a cascade of intracellular signaling pathways that mediate the chemotactic and other biological activities of IP-10 .

Recombinant IP-10 is widely used in research to study its role in various biological processes and diseases. For instance, it is used in chemotaxis assays to investigate its ability to induce cell migration . Additionally, IP-10 is employed in binding assays to study its interaction with other proteins, such as Insulin-Like Growth Factor Binding Protein 7 (IGFBP7) .

Recombinant IP-10 is typically supplied as a lyophilized powder and should be reconstituted with a suitable buffer, such as 20 mM Tris and 150 mM NaCl . For short-term storage, it can be kept at 4°C, while for long-term storage, it should be stored at -20°C or below .