IL 6 Mouse, Sf9
Description
Interleukin-6 Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 193 amino acids (25-211a.a.) and having a molecular mass of 22.5kDa (Molecular size on SDS-PAGE will appear at approximately 18-28kDa). IL6 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Interleukin-6 (IL-6) is a multifunctional cytokine that plays a crucial role in immune responses, inflammation, and hematopoiesis. It is produced by various cell types, including T cells, macrophages, fibroblasts, and endothelial cells. The recombinant form of IL-6, specifically from mouse origin and expressed in Sf9 insect cells, has been widely used in research to study its biological functions and potential therapeutic applications.
Interleukin-6 (Mouse Recombinant, Sf9) is produced using the baculovirus expression system in Sf9 insect cells. This recombinant protein is a single, glycosylated polypeptide chain containing 193 amino acids, with a molecular mass of approximately 22.5 kDa . The protein is expressed with a 6 amino acid His tag at the C-terminus, which facilitates its purification through chromatographic techniques .
IL-6 is a potent inducer of the acute phase response and has a wide range of biological activities:
- B-cell Differentiation: IL-6 plays a vital role in the final differentiation of B cells into immunoglobulin-secreting cells .
- Cell Proliferation: It induces the growth of myeloma and plasmacytoma cells .
- Nerve Cell Differentiation: IL-6 promotes the differentiation of nerve cells .
- Acute Phase Reactants: In hepatocytes, IL-6 induces the production of acute phase reactants .
The recombinant IL-6 protein is supplied as a sterile, filtered colorless solution. For short-term storage, it can be kept at 4°C for 2-4 weeks. For long-term storage, it is recommended to store the protein at -20°C with the addition of a carrier protein such as 0.1% HSA or BSA to prevent degradation . It is crucial to avoid multiple freeze-thaw cycles to maintain the protein’s stability and activity .
Recombinant IL-6 (Mouse, Sf9) is extensively used in various research applications, including:
- Immunological Studies: Understanding the role of IL-6 in immune responses and its potential as a therapeutic target for autoimmune diseases.
- Cancer Research: Investigating the involvement of IL-6 in cancer progression and its potential as a biomarker for certain types of cancer .
- Neuroscience: Studying the effects of IL-6 on nerve cell differentiation and its potential role in neurodegenerative diseases .
