IL 6 Human, His
Protein is >95% pure as determined by 10% PAGE (coomassie staining).
Description
IL-6 Human Recombinant produced in E.Coli migrates to 25kDa and is fused to a 6 amino acid his tag at its C-terminus. IL-6 is purified by proprietary chromatographic technique.
Product Specs
Product Science Overview
Interleukin-6 (IL-6) is a potent pro-inflammatory cytokine primarily produced by activated T cells, endothelial cells, and macrophages. It plays a crucial role in immune responses, acute phase reactions, and hematopoiesis. The recombinant form of IL-6, tagged with a His tag, is widely used in research to study its molecular characteristics and functions.
The human recombinant IL-6 with a His tag is produced in Escherichia coli (E. coli) and migrates to approximately 25 kDa. The His tag, consisting of six histidine residues, is fused to the C-terminus of the protein, facilitating its purification through affinity chromatography . The recombinant IL-6 is supplied as a sterile filtered clear solution in phosphate-buffered saline and 25 mM potassium carbonate .
IL-6 is involved in a wide range of biological activities, including:
- Immune Response: IL-6 affects both B and T lymphocytes, playing a pivotal role in host defense mechanisms .
- Acute Phase Reaction: It is a key mediator in the acute phase response, which is the body’s immediate reaction to infection or injury .
- Hematopoiesis: IL-6 influences the production and differentiation of blood cells .
- Inflammation: It is a major player in inflammatory processes and has been associated with various inflammatory diseases .
The recombinant IL-6 with a His tag is extensively used in immunological studies to explore its functions and interactions. It enables researchers to delve deeper into cytokine-mediated immune responses and understand the signaling pathways involved . The His tag allows for easy purification and detection of the protein, making it a valuable tool in laboratory research.
For short-term use, the recombinant IL-6 should be stored at 4°C. For long-term storage, it is recommended to keep it frozen at -20°C, with the addition of a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) to prevent degradation. Multiple freeze-thaw cycles should be avoided to maintain the protein’s integrity .
