IL 6 Human
Interleukin-6 Human Recombinant
Cat. No.
BT5965
Source
Escherichia Coli.
Synonyms
B cell differentiation factor, BCDF, BSF-2, HPGF, HSF, MGI-2, B-cell stimulatory factor 2,Hybridoma growth factor, CTL differentiation factor, CDF, IL-6, HGF.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Interleukin-6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 184 amino acids and having a molecular mass of 21000 Dalton.
The IL6 is purified by proprietary chromatographic techniques.
The IL6 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Interleukin 6 (IL-6) is a multifunctional cytokine that plays a role in various biological processes. It is crucial for the differentiation of B cells into antibody-secreting cells and influences the growth of myeloma and plasmacytoma cells. Additionally, IL-6 is involved in nerve cell differentiation and the induction of acute-phase reactants in hepatocytes.
Description
Recombinant Human Interleukin-6, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 184 amino acids. With a molecular weight of 21 kDa, this purified protein is obtained through proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The protein is lyophilized from a 0.2 μm filtered concentrated solution (1 mg/mL) in phosphate-buffered saline (PBS) at pH 7.4.
Solubility
To reconstitute the lyophilized Interleukin-6, it is recommended to dissolve it in sterile 18 MΩ-cm H2O to a concentration of at least 100 μg/mL. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized Interleukin-6 remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store the desiccated protein below -18°C. After reconstitution, store IL-6 at 4°C for 2-7 days. For future use, store below -18°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 97.0% as determined by two methods: Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis and Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
Biological Activity
The biological activity, represented by ED50 (half-maximal effective concentration), is determined by the dose-dependent stimulation of murine 7TD1 cells. The ED50 is less than 0.1 ng/mL, corresponding to a specific activity of 1.0 x 10,000,000 units per mg.
Protein Content
Protein quantification was performed using two independent methods. The first method involved UV spectroscopy at 280 nm, utilizing an absorbance value of 0.47 as the extinction coefficient for a 0.1% (1 mg/mL) solution. This value is calculated using the PC GENE computer analysis program (IntelliGenetics). The second method utilized RP-HPLC analysis with a standard solution of IL-6 as a reference standard.
Synonyms
B cell differentiation factor, BCDF, BSF-2, HPGF, HSF, MGI-2, B-cell stimulatory factor 2,Hybridoma growth factor, CTL differentiation factor, CDF, IL-6, HGF.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Pro-Val-Pro-Pro.
Product Science Overview
Structure and Production
Functions
IL-6 has several key functions:
- Immune Response: It promotes the differentiation of B and T cells, particularly Th17 cells .
- Inflammation: IL-6 is involved in the acute phase response and can induce fever in individuals with autoimmune diseases or infections .
- Hematopoiesis: It supports the proliferation of hematopoietic progenitor cells and hybridoma cells .
Clinical Significance
Applications
Recombinant human IL-6 is used in:
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