IL-6 Mouse, His
Protein is >95% pure as determined by 10% PAGE (coomassie staining).
Description
Interleukin-6 Mouse Recombinant produced in E.Coli migrates at 25kDa. Recombinant IL-6 Mouse is fused to a 6xHis tag at C-terminus and purified by proprietary chromatographic technique.
Product Specs
Interleukin-6, a potent inducer of inflammation, is primarily generated by activated T cells and various other cell types, such as endothelial cells and macrophages. This cytokine influences both B and T lymphocytes, playing a significant role in host defense mechanisms, acute phase responses, immune reactions, and the formation of blood cells (hematopoiesis).
Recombinant Mouse Interleukin-6, expressed in E. coli, exhibits a molecular weight of 25kDa. This protein is engineered with a 6xHis tag fused to its C-terminus and is purified using a proprietary chromatographic method.
The IL6 Mouse protein solution is supplied in a buffer consisting of 25mM Potassium Carbonate (K2CO3) and Phosphate Buffered Saline (PBS).
To ensure long-term stability, adding a carrier protein (0.1% HSA or BSA) is advisable.
Repeated freezing and thawing should be avoided.
The purity of the protein exceeds 95%, as determined by 10% SDS-PAGE analysis followed by Coomassie blue staining.
Product Science Overview
Interleukin-6 (IL-6) is a multifunctional cytokine that plays a crucial role in immune responses, inflammation, and hematopoiesis. It is primarily produced by activated T cells, endothelial cells, and macrophages. The recombinant form of IL-6, tagged with a His (histidine) tag, is widely used in research to study its functions and applications.
Interleukin-6 (Mouse Recombinant, His Tag) is produced in Escherichia coli (E. coli) as a single, non-glycosylated polypeptide chain. It consists of 208 amino acids and has a molecular mass of approximately 24 kDa. The His tag, which is a sequence of histidine residues, is fused to the N-terminus of the protein. This tag facilitates the purification and characterization of the protein using affinity chromatography techniques .
IL-6 is a potent pro-inflammatory cytokine that affects both B and T lymphocytes. It plays a significant role in host defense mechanisms, acute phase reactions, and immune responses. Some of the key functions of IL-6 include:
- Immune Cell Activation: IL-6 stimulates the activation and differentiation of B cells into antibody-producing plasma cells. It also promotes the differentiation of T cells and the production of other cytokines.
- Inflammation: IL-6 is involved in the inflammatory response by inducing the production of acute-phase proteins in the liver. It also contributes to the recruitment of immune cells to sites of infection or injury.
- Hematopoiesis: IL-6 supports the growth and differentiation of hematopoietic stem cells, which are responsible for the production of blood cells .
The recombinant form of IL-6, tagged with His, is a valuable tool in immunological research. It allows scientists to study the molecular intricacies of IL-6 and its impact on immune modulation. Some of the research applications include:
- Cytokine Signaling Pathways: Researchers use IL-6 (Mouse Recombinant, His Tag) to investigate the signaling pathways involved in cytokine production and response.
- Disease Models: IL-6 is used in various disease models to study its role in conditions such as autoimmune diseases, inflammatory disorders, and cancer.
- Therapeutic Development: Understanding the functions of IL-6 can aid in the development of targeted therapies for diseases where IL-6 plays a critical role .
