IL 6 Mouse

IL-6 was originally discovered in the media of cells stimulated with double-stranded RNA. It is produced by various cell types, including T cells, macrophages, fibroblasts, and endothelial cells . IL-6 is a potent inducer of the acute phase response and is involved in the body’s defense mechanisms following infection and injury .

IL-6 exerts its effects by binding to its receptor, IL-6Rα, which then associates with the signal-transducing component gp130. This interaction triggers the dimerization of gp130, leading to the activation of the Jak/Stat signaling pathway and the SHP2/MAPK (Erk) cascade . Additionally, IL-6 can form a complex with a soluble form of IL-6Rα, allowing it to activate gp130 signaling on cells that do not express IL-6Rα .

Recombinant mouse IL-6 is typically produced in E. coli and is used in various research applications, including cell culture, differentiation studies, and functional assays . The recombinant protein is often supplied in a lyophilized form and requires reconstitution with sterile water or a buffer containing a carrier protein such as BSA (Bovine Serum Albumin) to enhance stability and shelf-life .

Recombinant mouse IL-6 is widely used in scientific research to study its role in:

• Inflammation: IL-6 is a key mediator of the inflammatory response and is involved in the regulation of immune cell proliferation and differentiation.
• Hematopoiesis: IL-6 plays a role in the production and differentiation of blood cells.
• Cancer: IL-6 has been implicated in cancer progression, particularly in promoting angiogenesis and metastasis through the upregulation of proangiogenic factors like VEGF .

Recombinant mouse IL-6 should be stored at -20°C to -70°C to maintain its stability. Once reconstituted, it can be stored at 4°C for short-term use or at -20°C to -80°C for long-term storage. It is important to avoid repeated freeze-thaw cycles to prevent loss of bioactivity .