IL 6 Rhesus Macaque

Interleukin-6 (IL-6) is a multifunctional cytokine that plays a crucial role in immune response, inflammation, and hematopoiesis. The recombinant form of IL-6 derived from Rhesus macaques is particularly valuable for research due to its close genetic similarity to humans, making it an excellent model for studying human diseases and immune responses.

The IL-6 protein in Rhesus macaques is composed of 212 amino acids and has a molecular weight of approximately 21.1 kDa . It is typically expressed in Escherichia coli (E. coli) systems, which allows for high-yield production and easy purification . The recombinant IL-6 is often lyophilized for storage and can be reconstituted in sterile distilled water for experimental use .

IL-6 is known for its role in inducing B-cell differentiation and acting as a primary regulator of inflammation . It has been shown to regulate tumor proliferation and inhibit apoptosis, contributing to the progression of diseases such as coronary artery disease . The biological activity of recombinant IL-6 is measured using cell proliferation assays, with an effective dose (ED50) of less than 0.1 ng/mL, indicating its high potency .

Recombinant IL-6 from Rhesus macaques is used extensively in various research applications, including:

• Functional Studies: Investigating the role of IL-6 in immune responses and disease progression.
• SDS-PAGE: Analyzing the purity and molecular weight of the protein.
• Cell Proliferation Assays: Measuring the biological activity of IL-6 in promoting cell growth .

For optimal stability, recombinant IL-6 should be stored at 2°C to 8°C for short-term use and at -20°C to -80°C for long-term storage . It is important to aliquot the protein to avoid repeated freeze-thaw cycles, which can degrade its activity .