IL 21 Rat
Interleukin-21 Rat Recombinant
Cat. No.
BT2565
Source
Escherichia Coli.
Synonyms
Interleukin-21, IL-21, Il21.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Interleukin-21 Rat Recombinant produced in E.Coli is a single, non-glycosilated polypeptide chain containing 129 amino acids and having a total molecular mass of 15.2kDa.
The Rat IL-21 is purified by proprietary chromatographic techniques.
The Rat IL-21 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
IL-21, a cytokine produced by CD4+ T cells upon antigenic stimulation, plays a crucial role in augmenting antigen-specific immune responses. Its effects encompass promoting the differentiation of antigen-stimulated B-cells into plasma cells and memory B-cells, synergistically enhancing IgG production with IL-4, and inducing apoptosis in naive and stimulated B-cells in the absence of T-cell signaling. Moreover, IL-21 bolsters the anti-tumor activity of CD8+ T-cells and NK cells. It exerts these effects by binding to its specific type I cytokine receptor, IL-21R, which shares the common gamma chain (γC) with other cytokine receptors like IL-2, IL-4, IL-7, IL-9, and IL-15. This interaction sets off a signaling cascade, activating tyrosine kinases JAK1 and JAK3, followed by the activation of transcription factors STAT1 and STAT3.
Description
Recombinant Rat Interleukin-21, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 129 amino acids. With a molecular weight of 15.2 kDa, this protein is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The Rat IL-21 was lyophilized from a 0.2 µm filtered concentrated solution in PBS with a pH of 7.4.
Solubility
For reconstitution, it is recommended to dissolve the lyophilized Rat IL-21 in sterile 18 MΩ-cm H2O at a concentration not less than 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized Rat IL-21 remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store desiccated below -18°C. Once reconstituted, Rat IL-21 should be stored at 4°C for 2-7 days. For future use, it can be stored below -18°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 95.0% through the following methods: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Biological Activity
The biological activity is evaluated based on the protein's ability to stimulate the proliferation of activated B cells.
Synonyms
Interleukin-21, IL-21, Il21.
Source
Escherichia Coli.
Amino Acid Sequence
HKSSPQRPDH LLIRLRHLMD IVEQLKIYEN DLDPELLTAP QDVKGQCEHE AFACFQKAKL KPSNTGNNKT FINDLLAQLR RRLPAKRTGN KQRHMAKCPS CDLYEKKTPK EFLERLKWLL QKMIHQHLS.
Product Science Overview
Structure and Production
Recombinant IL-21, including the rat version, is typically produced using recombinant DNA technology. This involves inserting the gene encoding IL-21 into a suitable expression system, such as E. coli or mammalian cells, to produce the protein in large quantities. The recombinant protein is then purified to achieve high levels of purity, often exceeding 95% .
Biological Functions
IL-21 has several important biological functions:
- T Cell Activation and Proliferation: IL-21 enhances the proliferation and activation of T cells, which are essential for adaptive immunity.
- B Cell Differentiation: It promotes the differentiation of B cells into plasma cells, which produce antibodies.
- NK Cell Function: IL-21 enhances the cytotoxic activity of NK cells, which are crucial for the innate immune response .
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