Sf9, Baculovirus cells.
Interleukin-21 isoform 1, CVID11, IL-21, Za11.
Sterile filtered colorless solution.
Greater than 85.0% as determined by SDS-PAGE.
IL21 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 145 amino acids (30-162a.a.) and having a molecular mass of 16.9kDa. (Molecular size on SDS-PAGE will appear at approximately 18-28kDa).
IL21 is expressed with a 12 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Interleukin-21, also known as IL21, is a T helper cytokine that plays a role in regulating immune responses, hematopoiesis, and cell-mediated responses. It is essential for lymphocyte development and is produced by activated T cells. IL21 is also involved in the persistence of viral infections.
Recombinant human IL21, expressed in Sf9 insect cells, is a single, glycosylated polypeptide chain consisting of 145 amino acids (residues 30-162). It has a molecular weight of 16.9 kDa. Note that the protein's molecular size on SDS-PAGE may appear between 18-28 kDa. The protein contains a 12 amino acid His tag at its C-terminus and is purified using proprietary chromatographic methods.
The product is a sterile-filtered, colorless solution.
The IL21 protein is supplied as a 0.25 mg/ml solution in phosphate-buffered saline (pH 7.4) containing 20% glycerol.
For short-term storage (2-4 weeks), the product can be stored at 4°C. For longer storage, it is recommended to store the product frozen at -20°C. To ensure stability during long-term storage, adding a carrier protein such as 0.1% HSA or BSA is advised. Avoid repeated freeze-thaw cycles.
The purity of IL21 is greater than 85% as determined by SDS-PAGE analysis.
Interleukin-21 isoform 1, CVID11, IL-21, Za11.
Sf9, Baculovirus cells.
ADPEFMQGQD RHMIRMRQLI DIVDQLKNYV NDLVPEFLPA PEDVETNCEW SAFSCFQKAQ LKSANTGNNE RIINVSIKKL KRKPPSTNAG
RRQKHRLTCP SCDSYEKKPP KEFLERFKSL LQKMIHQHLS SRTHGSEDSH HHHHH.
Interleukin-21 (IL-21) is a cytokine that plays a crucial role in the regulation of the immune system. It is produced by activated CD4+ T cells and natural killer T (NKT) cells. IL-21 is involved in the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells, making it a key player in the body’s defense mechanisms against infections and diseases.
Recombinant human IL-21 produced in Sf9 cells is a single, glycosylated polypeptide chain containing 145 amino acids. The molecular mass of this recombinant protein is approximately 16.9 kDa, although it may appear as 18-28 kDa on SDS-PAGE due to glycosylation . The protein is expressed with a 12 amino acid His tag at the C-terminus, which facilitates its purification through chromatographic techniques .
IL-21 has several important functions in the immune system:
Recombinant IL-21 is used in various research applications, including:
The use of Sf9 cells, derived from the fall armyworm Spodoptera frugiperda, is a common method for producing recombinant proteins. These cells are infected with a baculovirus vector containing the gene of interest, in this case, human IL-21. The advantages of using Sf9 cells include high protein yield, proper folding, and post-translational modifications such as glycosylation, which are essential for the biological activity of the protein.