IL21 Canine
Escherichia Coli.
Interleukin-21.
Greater than 95.0% as determined by SDS-PAGE.
Description
IL21 Canine Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids (18-146a.a.) and having a molecular mass of 15kDa.
Product Specs
Interleukin-21.
Escherichia Coli.
MHKSSFQEQD LLLIRMRQLI DIVDQLKNYV NDLDPESLPA PEDVKRHCER SAFSCFQKVQ LKAANTGGNE QIINVLTKQL KRKLPPTNAG RRQKHRPACP SCDSYEKAPP KEFLERLKSL IQKMIHQHLS
Product Science Overview
Recombinant Canine IL-21 is produced using Escherichia coli (E. coli) as the expression host. The recombinant protein typically includes an N-terminal methionine and spans from His18 to Ser146 . The molecular mass of the recombinant canine IL-21 is approximately 15 kDa, and it is purified to a high degree, often exceeding 95% purity as determined by SDS-PAGE .
IL-21 has significant immunoregulatory activity. It enhances the secretion of interferon-gamma (IFN-γ) in NK-92 human natural killer lymphoma cells, with an effective dose (ED50) of around 8 ng/mL . This cytokine is involved in the proliferation and differentiation of T cells, B cells, and NK cells, making it a critical component in both innate and adaptive immunity .
Recombinant Canine IL-21 is used in various research applications, including:
The recombinant protein is typically lyophilized and can be reconstituted in PBS (phosphate-buffered saline). It is stable for up to 12 months when stored at -20°C to -70°C. After reconstitution, it should be stored under sterile conditions at 2°C to 8°C for up to one month or at -20°C to -70°C for up to three months .
