IL 17F His Human

Interleukin-17F Human Recombinant, His Tag
Cat. No.
BT31031
Source
Escherichia Coli.
Synonyms
Cytokine ML-1, IL-17F, Interleukin-17F precursor, IL17F, ML1, ML-1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

IL 17F His Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 158 amino acids (31-163) and having a molecular mass of 17.6kDa.
IL 17F His is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
IL-17F, classified as Q96PD4, is a cytokine that shares structural similarities with IL-17. Primarily produced by activated T cells, IL-17F stimulates the production of other cytokines such as IL6, IL8, and CSF2/GM_CSF. Furthermore, IL-17F exhibits anti-angiogenic properties in endothelial cells and triggers them to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. In stromal cells, IL-17F induces the production of proinflammatory and hematopoietic cytokines. Notably, there's an increase in intestinal IL17F gene expression in active Crohn's Disease. Studies reveal that variations in IL-17A & IL-17F alleles independently influence susceptibility to and the pathological characteristics of ulcerative colitis. Additionally, polymorphisms in the IL-17F and MIF genes are significantly linked to the development of functional dyspepsia. Mechanistically, the IL-17F/IL-17R signaling pathway is initiated by TRAF6-mediated receptor ubiquitination. IL-17F also induces the expression of IFN-gamma-inducible protein 10 (IP-10) by activating the Raf1-mitogen-activated protein kinase 1/2-extracellular-regulated kinase 1/2-p90 ribosomal S6 kinase-cyclic AMP response element-binding protein signaling pathway.
Description
Recombinant Human IL-17F His, produced in E.coli, is a single, non-glycosylated polypeptide chain comprising 158 amino acids (31-163). It possesses a molecular weight of 17.6kDa. The protein includes a 25 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The IL 17F His solution is provided at a concentration of 1mg/ml and contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea, and 10% glycerol.
Stability
For optimal storage, keep at 4°C if the entire vial will be used within 2-4 weeks. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. It is crucial to avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 85% as assessed by SDS-PAGE analysis.
Synonyms
Cytokine ML-1, IL-17F, Interleukin-17F precursor, IL17F, ML1, ML-1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMRKIPK VGHTFFQKPE SCPPVPGGSM KLDIGIINEN QRVSMSRNIE SRSTSPWNYT VTWDPNRYPS EVVQAQCRNL GCINAQGKED ISMNSVPIQQ ETLVVRRKHQ GCSVSFQLEK VLVTVGCTCV TPVIHHVQ

Product Science Overview

Introduction

Interleukin-17F (IL-17F) is a cytokine that plays a crucial role in the immune system. It is part of the IL-17 family, which includes several other members such as IL-17A, IL-17B, IL-17C, IL-17D, and IL-17E (also known as IL-25). IL-17F is known for its involvement in inflammatory responses and has been implicated in various autoimmune diseases.

Structure and Expression

IL-17F is a secreted disulfide-linked homodimer, meaning it consists of two identical subunits linked by disulfide bonds . It is expressed in activated CD4+ T-cells and activated monocytes, but not in resting cells . The recombinant form of IL-17F, tagged with a polyhistidine (His) tag, is produced in human 293 cells (HEK293) and is used for various research purposes .

Biological Functions

IL-17F has several important biological functions:

  • Cytokine Production: It stimulates the production of other cytokines, including IL-6, IL-8, and granulocyte colony-stimulating factor .
  • Regulation of Cartilage Matrix: IL-17F can regulate cartilage matrix turnover, which is crucial for maintaining joint health .
  • Cell Proliferation: It stimulates the proliferation of peripheral blood mononuclear cells (PBMCs) and T-cells .
  • Angiogenesis Inhibition: IL-17F inhibits the angiogenesis of endothelial cells and induces them to produce IL-2, TGF-β1, and monocyte chemoattractant protein-1 .
Clinical Significance

Defects in IL-17F are associated with familial candidiasis type 6 (CANDF6), a condition characterized by chronic mucocutaneous candidiasis . This highlights the importance of IL-17F in maintaining immune homeostasis and protecting against fungal infections.

Recombinant IL-17F (His Tag)

The recombinant form of IL-17F with a His tag is used extensively in research. The His tag allows for easy purification and detection of the protein. This recombinant protein is typically lyophilized and can be reconstituted for use in various assays . It is stable for long-term storage when kept at -20°C or lower and should be handled carefully to avoid repeated freeze-thaw cycles .

Applications

Recombinant IL-17F (His Tag) is used in various research applications, including:

  • ELISA: To measure the concentration of IL-17F in biological samples .
  • Binding Studies: To study the interaction between IL-17F and its receptors .
  • Functional Assays: To investigate the biological activities of IL-17F, such as its effects on cell proliferation and cytokine production .

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