Introduction
IL-17F, identified by the accession number Q96PD4, is a cytokine that exhibits sequence homology with IL-17. Primarily produced by activated T cells, IL-17F has been observed to induce the production of various cytokines, including IL-6, IL-8, and CSF2/GM-CSF. This cytokine demonstrates inhibitory effects on the angiogenesis of endothelial cells while promoting their production of IL-2, TGFB1/TGFB, and monocyte chemoattractant protein-1. Furthermore, IL-17F stimulates stromal cells to produce proinflammatory and hematopoietic cytokines. Notably, intestinal IL-17F gene expression is upregulated in active Crohn's disease. Studies suggest that IL-17A and IL-17F alleles independently influence susceptibility to and the pathophysiological characteristics of ulcerative colitis. Additionally, polymorphisms in IL-17F and MIF genes are significantly associated with the development of functional dyspepsia. The initiation of the IL-17F/IL-17R signaling pathway is contingent upon receptor ubiquitination by TRAF6. IL-17F activates the Raf1-mitogen-activated protein kinase 1/2-extracellular-regulated kinase 1/2-p90 ribosomal S6 kinase-cyclic AMP response element-binding protein signaling pathway, thereby inducing the expression of IFN-gamma-inducible protein 10 (IP-10).
Description
Recombinant Human Interleukin-17F, produced in E. coli, is a homodimeric protein consisting of two polypeptide chains, each composed of 134 amino acids. The chains are linked by cysteine residues and lack glycosylation. The total molecular weight of the protein is 30.1 kDa. Purification of Human IL-17F is achieved through proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White Lyophilized Powder
Formulation
Lyophilized from a 1 mg/mL solution in 10 mM sodium citrate buffer, pH 3.
Solubility
To reconstitute lyophilized Human Interleukin-17F, it is recommended to dissolve the powder in sterile water to a concentration not exceeding 1 mg/mL. This solution can then be further diluted in other aqueous solutions as needed.
Stability
Lyophilized Human Interleukin-17F remains stable at room temperature for up to 3 weeks; however, it is recommended to store the desiccated product at a temperature below -18°C for optimal long-term stability. After reconstitution, Human IL-17F should be stored at 4°C for no longer than 2-7 days. For extended storage, it is advisable to store the reconstituted product at -18°C. To further enhance stability during long-term storage, the addition of a carrier protein such as 0.1% HSA or BSA is recommended. Repeated freeze-thaw cycles should be avoided.
Purity
Greater than 97.0% as determined by: (a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis. (b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Synonyms
Cytokine ML-1, IL-17F, Interleukin-17F precursor, IL17F, ML1, ML-1.
Amino Acid Sequence
MRKIPKVGHT FFQKPESCPP VPGGSMKLDI GIINENQRVS MSRNIESRST SPWNYTVTWD PNRYPSEVVQ AQCRNLGCIN AQGKEDISMN SVPIQQETLV VRRKHQGCSV SFQLEKVLVT VGCTCVTPVI HHVQ.
