IL17D Human

Interleukin-17D Human Recombinant
Cat. No.
BT9563
Source
Escherichia Coli.
Synonyms
Interleukin 17D, Interleukin 27, IL-17D, IL-27, IL27, Interleukin-17D, Interleukin-27, IL17D.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Interleukin-17D Human Recombinant (18-202) produced in E.Coli is a non-glycosylated disulfide-linked homodimer containing 2 polypeptide chains of 185 amino acids each and having a molecular mass of 40kDa.
The IL-17D is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Interleukin 17D (IL-17D) is a cytokine that plays a role in regulating immune responses. It stimulates the production of molecules involved in inflammation and immunity, such as myeloid growth factors and chemokines. IL-17D also has a role in suppressing the growth of myeloid progenitor cells, which are cells that can develop into various types of blood cells. IL-17D is found in various tissues, including skeletal muscle, heart, adipose tissue, lung, pancreas, and nervous system. It shares structural similarities with IL-17B, another member of the IL-17 family. When IL-17D interacts with endothelial cells, it triggers the production of other signaling molecules like IL-6, IL-8, and CSF2/GM-CSF, contributing to the immune response. This increased production of IL-8 is regulated by a protein complex called NF-kappa B.
Description
This product is a recombinant human IL-17D protein produced in E. coli bacteria. It is a non-glycosylated, disulfide-linked homodimer, meaning it is made up of two identical protein chains, each containing 185 amino acids. The total molecular weight of the protein is 40 kDa. The protein has been purified using specific chromatographic methods to ensure its purity.
Physical Appearance
White powder, freeze-dried and sterilized by filtration.
Formulation
The protein was freeze-dried from a solution containing acetonitrile and TFA that had been passed through a 0.2 micrometer filter.
Solubility
To reconstitute the freeze-dried IL-17D, it is recommended to dissolve it in sterile 18 megaohm-cm water to a concentration of at least 100 micrograms per milliliter. This solution can then be further diluted in other aqueous solutions as needed.
Stability
The lyophilized IL-17D can be stored at room temperature for up to 3 weeks; however, it is recommended to store it in a dry environment below -18 degrees Celsius for extended periods. Once reconstituted, the IL-17D should be stored at 4 degrees Celsius for up to 7 days. For long-term storage, it is advisable to add a carrier protein like HSA or BSA (0.1%) and store it below -18 degrees Celsius. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 97% as determined by SDS-PAGE analysis.
Biological Activity
The recombinant human IL-17D protein exhibits biological activity by binding to the recombinant human IL-17BR protein with an EC50 (half maximal effective concentration) of less than 2 micrograms per milliliter in a binding assay.
Synonyms
Interleukin 17D, Interleukin 27, IL-17D, IL-27, IL27, Interleukin-17D, Interleukin-27, IL17D.
Source
Escherichia Coli.
Amino Acid Sequence
APRAGRRPAR PRGCADRPEE LLEQLYGRLA AGVLSAFHHT LQLGPREQAR NASCPAGGRP ADRRFRPPTN LRSVSPWAYR ISYDPARYPR YLPEAYCLCR GCLTGLFGEE DVRFRSAPVY MPTVVLRRTP ACAGGRSVYT EAYVTIPVGC TCVPEPEKDA DSINSSIDKQ GAKLLLGPND APAGP.

Product Science Overview

Structure and Expression

IL-17D is a secreted cytokine with a conserved cysteine-knot fold near the C-terminus, similar to other IL-17 family members . It is expressed in various tissues, including skeletal muscle, heart, adipose tissue, lung, pancreas, and the nervous system . The recombinant form of IL-17D is typically produced in E. coli and has a predicted molecular mass of approximately 20 kDa per monomer .

Biological Functions

IL-17D modulates immune responses indirectly by stimulating the production of myeloid growth factors and chemokines . It has been shown to stimulate the production of cytokines such as IL-6, IL-8, and GM-CSF in endothelial cells . Additionally, IL-17D has an inhibitory effect on the hemopoiesis of myeloid progenitor cells .

Role in Disease and Therapeutic Potential

The IL-17 family, including IL-17D, has been linked to various disease processes such as rheumatoid arthritis, asthma, lupus, allograft rejection, tumorigenicity, and antitumor immunity . IL-17D, in particular, has been found to be highly secreted by fibrosarcoma tumor cells and can recruit natural killer cells via the production of CCL2 by endothelial cells .

Applications of Recombinant IL-17D

Recombinant IL-17D is used in research to study its role in immune responses and its potential therapeutic applications. It is available in both carrier-free and carrier-containing formulations, with the carrier protein typically being Bovine Serum Albumin (BSA) to enhance protein stability . The recombinant protein is lyophilized and can be reconstituted for use in various experimental setups .

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