IL17A Human, Sf9

Interleukin 17A Human Recombinant, Sf9
Cat. No.
BT9227
Source
Sf9, Baculovirus cells.
Synonyms
Interleukin 17A, CTLA8, IL17, Interleukin 17 (Cytotoxic T-Lymphocyte-Associated Serine Esterase 8), Cytotoxic T-Lymphocyte-Associated Protein 8, Cytotoxic T-Lymphocyte-Associated Antigen 8, CTLA-8, IL-17A, IL-17, Interleukin-17A.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

IL17A produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (24-155 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 138 amino acids and having a molecular mass of 15.9kDa.
IL17A shows multiple bands between 13.5-28kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Interleukin 17 (IL-17), also known as IL-17A, is a pro-inflammatory cytokine that plays a critical role in immune responses, particularly in the context of inflammation. It is primarily produced by a subset of T helper cells called Th17 cells. IL-17 exerts its effects by binding to the IL-17 receptor, which is expressed on various cell types, including epithelial cells, fibroblasts, and immune cells. This binding triggers downstream signaling pathways, leading to the production of other inflammatory mediators, such as cytokines (e.g., IL-6), chemokines (e.g., CXCL8), and matrix metalloproteinases. Elevated levels of IL-17 have been implicated in the pathogenesis of various autoimmune and inflammatory diseases, including rheumatoid arthritis, psoriasis, and multiple sclerosis.
Description
Recombinant human IL-17A protein expressed in Sf9 insect cells using a baculovirus expression system. This protein is a single, glycosylated polypeptide chain containing amino acids 24-155 of the mature human IL-17A sequence and a C-terminal 6-His tag. The molecular weight of the protein is approximately 15.9 kDa, and it appears as multiple bands between 13.5-28 kDa on SDS-PAGE under reducing conditions. The protein has been purified using proprietary chromatographic techniques.
Physical Appearance
A clear and colorless solution that has been sterilized by filtration.
Formulation
The IL-17A protein is supplied as a solution with a concentration of 0.25 mg/ml in a buffer containing phosphate-buffered saline (PBS) at pH 7.4, 20% glycerol, 1 mM EDTA, and 0.1 mM PMSF.
Stability
For short-term storage (up to 2-4 weeks), the IL-17A protein can be stored at 4°C. For long-term storage, it is recommended to store the protein in aliquots at -20°C. To further enhance stability during long-term storage, adding a carrier protein such as 0.1% HSA or BSA is advised. Avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of IL-17A is determined by SDS-PAGE analysis and is guaranteed to be greater than 90%.
Biological Activity

The biological activity of IL-17A is measured by its ability to stimulate IL-6 production in NIH/3T3 mouse embryonic fibroblast cells. The ED50, which represents the concentration of IL-17A required to achieve half-maximal IL-6 production, is within the range of ≤7 ng/ml. IL-6 levels are determined using an enzyme-linked immunosorbent assay (ELISA).

Synonyms
Interleukin 17A, CTLA8, IL17, Interleukin 17 (Cytotoxic T-Lymphocyte-Associated Serine Esterase 8), Cytotoxic T-Lymphocyte-Associated Protein 8, Cytotoxic T-Lymphocyte-Associated Antigen 8, CTLA-8, IL-17A, IL-17, Interleukin-17A.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
GITIPRNPGC PNSEDKNFPR TVMVNLNIHN RNTNTNPKRS SDYYNRSTSP WNLHRNEDPE RYPSVIWEAK CRHLGCINAD GNVDYHMNSV PIQQEILVLR REPPHCPNSF RLEKILVSVG CTCVTPIVHH VAHHHHHH

Product Science Overview

Introduction

Interleukin 17A (IL-17A) is a proinflammatory cytokine produced by activated T cells. It plays a crucial role in the immune response by regulating the activities of NF-kappaB and mitogen-activated protein kinases. IL-17A can stimulate the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17A are associated with several chronic inflammatory diseases, including rheumatoid arthritis, psoriasis, and multiple sclerosis .

Production and Characteristics

The recombinant form of IL-17A, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain containing 138 amino acids and having a molecular mass of 15.9 kDa. This form of IL-17A is fused to a 6 amino acid His Tag at the C-terminus. The protein shows multiple bands between 13.5-28 kDa on SDS-PAGE under reducing conditions and is purified using proprietary chromatographic techniques .

Biological Activity

IL-17A is known for its proinflammatory properties. It regulates the activities of NF-kappaB and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO). These activities make IL-17A a key player in the immune response and inflammation .

Applications

Recombinant IL-17A is used in various research applications, particularly in studies related to inflammation and immune response. It is also used to investigate the role of IL-17A in chronic inflammatory diseases and to develop potential therapeutic interventions .

Storage and Stability

IL-17A protein solution should be stored at 4°C if it will be used within 2-4 weeks. For longer periods, it should be stored frozen at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA) to avoid multiple freeze-thaw cycles. The protein is stable and maintains its biological activity under these storage conditions .

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