IL 17A/F Rat

Interleukin-17A/F Heterodimer Rat Recombinant
Cat. No.
BT31013
Source
Escherichia Coli.
Synonyms
IL17A/F, IL17 A/F, IL-17A/F, IL-17 A/F, IL17AF, IL-17 AF, Interleukin-17 A/F, Interleukin-17 AF.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by HPLC and SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

IL-17A/F Rat Recombinant produced in E.Coli is a heterodimeric, non-glycosylated polypeptide chain containing 1 monomeric subunit of each IL-17A & IL-17F. The dimer contains 269 amino acids and having a total molecular mass of 30.7 kDa.
The IL-17A/F Rat is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Rat IL-17A/F is a heterodimeric glycoprotein composed of two IL-17 family members, IL-17A and IL-17F, linked by disulfide bonds. The IL-17 family consists of six homodimers, each characterized by a cysteine knot motif with two disulfide bonds. Rat IL-17A is initially synthesized as a 155 amino acid precursor, containing a 23 amino acid signal sequence and a 132 amino acid mature peptide with an N-linked glycosylation site. Similarly, rat IL-17F is produced as a 153 amino acid precursor, comprising a 20 amino acid signal sequence and a 133 amino acid mature peptide, also featuring an N-linked glycosylation site. IL-17A and IL-17F share 50% amino acid sequence identity. Human IL-17A and IL-17F exhibit approximately 60% homology to their murine counterparts. IL-17A/F, along with IL-17A and IL-17F homodimers, are produced by activated CD4+ T cells, specifically Th17 cells, in response to IL-23 stimulation. The IL-17RA and IL-17RC receptors form a heterodimer that binds IL-17A and IL-17F, with IL-17A/F demonstrating specific binding to IL-17RA. Functionally, IL-17A/F exhibits intermediate potency in inducing chemokine production and airway neutrophilia, compared to the most potent IL-17A and the least potent IL-17F.
Description
Recombinant Rat IL-17A/F, expressed in E. coli, is a non-glycosylated heterodimer composed of one monomer each of IL-17A and IL-17F. This dimer, comprising 269 amino acids, has a molecular weight of 30.7 kDa. Purification of Rat IL-17A/F is achieved using proprietary chromatographic methods.
Physical Appearance
White lyophilized powder, sterile filtered.
Formulation
Lyophilized from a 1 mg/ml solution without any additives.
Solubility
To reconstitute lyophilized Rat IL-17A/F, it is recommended to dissolve it in sterile water to a maximum concentration of 1 mg/ml. This solution can then be further diluted in other aqueous solutions as needed.
Stability
Lyophilized Rat IL-17A/F remains stable at room temperature for up to 3 weeks; however, it is recommended to store it desiccated at temperatures below -18°C. Once reconstituted, Rat IL-17A/F should be stored at 4°C for 2-7 days. For long-term storage, freezing below -18°C is advised. To enhance stability during long-term storage, consider adding a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 98.0% as determined by HPLC and SDS-PAGE analysis.
Biological Activity
The biological activity, assessed by the ability to induce IL-6 production in NIH-3T3 cells, is represented by an ED50 value ranging from 15.3 to 23 ng/ml.
Synonyms
IL17A/F, IL17 A/F, IL-17A/F, IL-17 A/F, IL17AF, IL-17 AF, Interleukin-17 A/F, Interleukin-17 AF.
Source
Escherichia Coli.
Amino Acid Sequence
IL-17A: MAVLIPQSSV CPNAEANNFL QNVKVNLKVL NSLSSKASSR RPSDYLNRST SPWTLSRNED PDRYPSVIWE AQCRHQRCVN AEGKLDHHMN SVLIQQEILV LKREPEKCPF TFRVEKMLVG VGCTCVSSIV RHAS.
IL-17F: MARRNPKVGL SALQKAGNCP PLEDNSVRVD IRIFNQNQGI SVPRDFQNRS SSPWDYNITR DPDRFPSEIA EAQCRHSGCI NAQGQEDGSM NSVPIQQEIL VLRREPQGCS NSFRLEKMLI KVGCTCVTPI VHHAA.

Product Science Overview

Introduction

Interleukin-17 (IL-17) is a family of pro-inflammatory cytokines that play a crucial role in the immune response. Among the IL-17 family, IL-17A and IL-17F are particularly significant due to their involvement in various inflammatory and autoimmune diseases. The IL-17A/F heterodimer is a unique combination of these two cytokines, exhibiting distinct biological activities compared to their homodimeric forms.

Structure and Production

The IL-17A/F heterodimer is a disulfide-linked glycosylated protein composed of IL-17A and IL-17F subunits. In the case of the recombinant rat IL-17A/F heterodimer, it is produced using Chinese Hamster Ovary (CHO) cells. The recombinant protein is typically purified to a high degree of purity (>95%) and is free from endotoxins (<0.10 EU per 1 μg of the protein) .

Biological Activity

The IL-17A/F heterodimer is biologically active and has been shown to induce the secretion of IL-6, a pro-inflammatory cytokine, in NIH-3T3 mouse embryonic fibroblast cells. The effective dose (ED50) for this activity ranges from 3 to 18 ng/mL . This activity is intermediate between the more potent IL-17A and the less potent IL-17F, highlighting the unique functional properties of the heterodimer.

Role in Inflammation and Disease

IL-17A and IL-17F are known to be upregulated in various inflammatory conditions, including autoimmune diseases such as rheumatoid arthritis and psoriasis. The IL-17A/F heterodimer contributes to the pathogenesis of these diseases by promoting the recruitment and activation of neutrophils and other immune cells, leading to tissue inflammation and damage .

Applications in Research

The recombinant rat IL-17A/F heterodimer is widely used in research to study its role in inflammation and immune responses. It serves as a valuable tool for investigating the signaling pathways and cellular mechanisms mediated by IL-17A and IL-17F. Additionally, it is used in the development of therapeutic strategies targeting IL-17-mediated diseases.

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