IL17F Rat

Interleukin-17F Rat Recombinant
Cat. No.
BT10118
Source
Escherichia Coli.
Synonyms
Cytokine ML-1, IL-17F, Interleukin-17F precursor, IL17F, ML1, ML-1.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

IL17F Rat Recombinant produced in E.Coli is a homodimeric, non-glycosylated polypeptide chain containing a total of 270 amino acids and having a molecular mass of 30 kDa.
The Rat IL-17F is purified by proprietary chromatographic techniques.

Product Specs

Introduction
IL-17F, identified by the accession number Q96PD4, is a cytokine that exhibits sequence similarity to IL-17. Primarily produced by activated T cells, IL-17F stimulates the production of various cytokines, including IL-6, IL-8, and CSF2/GM-CSF. Furthermore, IL-17F demonstrates the ability to inhibit angiogenesis in endothelial cells while promoting the production of IL-2, TGFB1/TGFB, and monocyte chemoattractant protein-1 in these cells. IL-17F also induces stromal cells to produce proinflammatory and hematopoietic cytokines. Elevated intestinal IL-17F gene expression is observed in active Crohn's disease. Studies indicate that IL-17A and IL-17F alleles independently influence susceptibility to and the pathophysiological characteristics of ulcerative colitis. Moreover, polymorphisms in the IL-17F and MIF genes are significantly linked to the development of functional dyspepsia. The activation of the IL-17F/IL-17R signaling pathway relies on TRAF6-mediated receptor ubiquitination. IL-17F induces the expression of IFN-gamma-inducible protein 10 (IP-10) by activating the Raf1-mitogen-activated protein kinase 1/2-extracellular-regulated kinase 1/2-p90 ribosomal S6 kinase-cyclic AMP response element-binding protein signaling pathway.
Description
Recombinant Rat IL17F, produced in E. coli, is a homodimeric, non-glycosylated polypeptide chain comprising 270 amino acids. It has a molecular weight of 30 kDa. The purification of Rat IL-17F is achieved using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Rat IL17F was lyophilized from a 1 mg/ml solution without any additives.
Solubility
To reconstitute the lyophilized Rat IL17F, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. This solution can then be further diluted into other aqueous solutions as needed.
Stability
Lyophilized Rat IL17F remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated below -18°C. After reconstitution, Rat IL17F should be stored at 4°C for 2-7 days. For future use, it should be stored below -18°C. To ensure long-term stability, it is advisable to add a carrier protein (0.1% HSA or BSA) and avoid repeated freeze-thaw cycles.
Purity
The purity of Rat IL17F is greater than 97.0% as determined by: (a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis and (b) SDS-PAGE analysis.
Synonyms
Cytokine ML-1, IL-17F, Interleukin-17F precursor, IL17F, ML1, ML-1.
Source
Escherichia Coli.
Amino Acid Sequence
MARRNPKVGL SALQKAGNCP PLEDNSVRVD IRIFNQNQGI SVPRDFQNRS SSPWDYNITR DPDRFPSEIA EAQCRHSGCI NAQGQEDGSM NSVPIQQEIL VLRREPQGCS NSFRLEKMLI KVGCTCVTPI VHHAA.

Product Science Overview

Introduction

Interleukin-17F (IL-17F) is a cytokine that belongs to the IL-17 family, which consists of six members: IL-17A, IL-17B, IL-17C, IL-17D, IL-17E (also known as IL-25), and IL-17F . IL-17F shares the greatest homology with IL-17A and is produced by several cell types, including activated CD4+ T cells, monocytes, basophils, and mast cells .

Structure and Expression

IL-17F is a protein that is encoded by the IL17F gene. The recombinant form of IL-17F, specifically from rats, is often produced using baculovirus-insect cell expression systems . The protein sequence of rat IL-17F consists of 153 amino acids, with a calculated molecular weight of approximately 16.4 kDa .

Biological Functions

IL-17F plays a crucial role in inducing and mediating pro-inflammatory responses. It stimulates the production of other cytokines, such as IL-6 and IL-8, and granulocyte colony-stimulating factor . Additionally, IL-17F has been shown to inhibit angiogenesis in endothelial cells and induce these cells to produce IL-2, TGF-β, and monocyte chemoattractant protein-1 .

Role in Diseases

The IL-17 family, including IL-17F, is involved in various physiological and pathological processes. IL-17F is associated with allergic responses and has been implicated in the exacerbation of antigen-induced pulmonary allergic inflammation . Moreover, IL-17F plays a role in the induction of neutrophilia in the lungs .

Research and Applications

Recombinant IL-17F is used extensively in research to study its biological functions and its role in various diseases. It is particularly valuable in understanding the mechanisms underlying inflammatory responses and developing potential therapeutic strategies for autoimmune and inflammatory diseases .

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