IL 17 Rat

Interleukin-17 Rat Recombinant
Cat. No.
BT31000
Source
Escherichia Coli.
Synonyms
CTLA-8, IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8, Interleukin-17A.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Interleukin-17A Rat Recombinant produced in E.Coli is a homodimeric, non-glycosylated polypeptide chain having a molecular mass of 30 kDa.
The IL-17A Rat is purified by proprietary chromatographic techniques.

Product Specs

Introduction
IL-17, or Interleukin-17, is a pro-inflammatory cytokine primarily secreted by activated T cells. It plays a crucial role in immune responses, particularly in inflammatory processes. IL-17 exerts its effects by binding to its receptor, leading to the activation of signaling pathways such as NF-kappaB and MAP kinases. These pathways regulate the expression of various genes involved in inflammation, including those encoding cytokines like IL-6, enzymes like COX-2 (involved in prostaglandin synthesis), and nitric oxide synthase (responsible for nitric oxide production). Elevated levels of IL-17 are implicated in the pathogenesis of numerous chronic inflammatory conditions, including rheumatoid arthritis, psoriasis, and multiple sclerosis.
Description
Recombinant Rat Interleukin-17A, produced in E. coli, is a non-glycosylated polypeptide arranged as a homodimer, with a molecular weight of 30 kDa. The purification process for this recombinant protein involves proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The product is lyophilized from a concentrated solution (1mg/ml) containing 10mM NaCitrate buffer at pH 3.0.
Solubility
To reconstitute the lyophilized Interleukin-17A Rat, it is recommended to dissolve it in sterile 18MΩ-cm H2O to a concentration of at least 100µg/ml. This solution can then be further diluted in other aqueous solutions as needed.
Stability
Lyophilized Interleukin-17A Rat, though stable at room temperature for up to 3 weeks, should ideally be stored in a dry environment below -18°C. Upon reconstitution, it's recommended to store Rat IL-17 at 4°C for short-term use (2-7 days) and at -18°C for long-term storage. For extended storage, adding a carrier protein like HSA or BSA (0.1%) is advisable. Avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of the product exceeds 97.0%, as confirmed by: (a) Reverse-phase High-Performance Liquid Chromatography (RP-HPLC) analysis, and (b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
Biological Activity
The biological activity of the product, measured as its effective dose (ED50), is determined by its ability to induce IL-6 production in mouse NIH 3T3 fibroblasts. The ED50 for this effect ranges from 0.44 to 0.66 ng/ml.
Synonyms
CTLA-8, IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8, Interleukin-17A.
Source
Escherichia Coli.
Amino Acid Sequence
MAVLIPQSSV CPNAEANNFL QNVKVNLKVL NSLSSKASSR RPSDYLNRST SPWTLSRNED PDRYPSVIWE AQCRHQRCVN AEGKLDHHMN SVLIQQEILV LKREPEKCPF TFRVEKMLVG VGCTCVSSIV RHAS.

Product Science Overview

Introduction

Interleukin-17 (IL-17) is a family of pro-inflammatory cytokines that play a crucial role in the immune response. The IL-17 family consists of six members: IL-17A, IL-17B, IL-17C, IL-17D, IL-17E, and IL-17F . Among these, IL-17A is the most studied and is often referred to simply as IL-17. IL-17A is produced by a subset of T helper cells known as Th17 cells and is involved in the defense against bacterial and fungal infections .

Discovery and Structure

IL-17A was first discovered in 1993 and was initially termed cytotoxic T lymphocyte antigen 8 (CTLA-8) . It is a glycosylated cytokine with a molecular weight ranging from 15 to 20 kDa . The IL-17 family members share a conserved cystine knot fold, which is crucial for their biological activity .

Biological Functions

IL-17A is a potent inducer of inflammatory responses. It promotes the maturation of CD34-positive hematopoietic precursors into neutrophils and stimulates the production of other pro-inflammatory cytokines and chemokines . This cytokine plays a significant role in the pathogenesis of various inflammatory and autoimmune diseases, including rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection .

Recombinant IL-17 (Rat)

Recombinant IL-17 (Rat) is a laboratory-produced version of the natural cytokine, designed for research purposes. It is used to study the biological functions of IL-17 in various experimental models. The recombinant protein retains the biological activity of the native cytokine and is used in studies related to inflammation, immune response, and disease pathogenesis .

Clinical Significance

IL-17A has been identified as a therapeutic target in several diseases due to its role in promoting inflammation. Inhibitors of IL-17A or its receptor (IL-17RA) are being developed and tested for the treatment of autoimmune and inflammatory conditions . Understanding the functions and mechanisms of IL-17A is crucial for developing new therapeutic strategies.

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