Sf9, Baculovirus cells.
IL6, IL-6, Interleukin-6.
IL6 Canine Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 195 amino acids (21-207a.a.) and having a molecular mass of 22.0kDa (Molecular size on SDS-PAGE will appear at approximately 18-28kDa).
IL6 is expressed with an 8 amino acid His-tag at C-Terminus and purified by proprietary chromatographic techniques.
IL6, IL-6, Interleukin-6.
Sf9, Baculovirus cells.
FPTPGPLAGD SKDDATSNSL PLTSANKVEE LIKYILGKIS ALRKEMCDKF NKCEDSKEAL AENNLHLPKL EGKDGCFQSG FNQETCLTRI TTGLVEFQLH LNILQNNYEG DKENVKSVHM STKILVQMLK SKVKNQDEVT TPDPTTDASL QAILQSQDEC VKHTTIHLIL RSLEDFLQFS LRAVRIMLEH HHHHH.
IL-6 is a glycoprotein with a molecular weight ranging from 22 to 28 kDa, depending on its glycosylation status . It is composed of four alpha-helices arranged in a bundle, a common structure among cytokines. IL-6 binds to its receptor, IL-6R, which then associates with the signal-transducing component gp130. This complex initiates intracellular signaling pathways, including the JAK/STAT, MAPK, and PI3K/Akt pathways .
In canines, IL-6 has similar functions to those in humans. It is involved in the regulation of immune responses, inflammation, and hematopoiesis. Canine IL-6 is particularly important in veterinary medicine for understanding and managing inflammatory diseases, infections, and immune-mediated conditions .
Recombinant Canine IL-6 is a laboratory-produced version of the natural cytokine. It is typically produced using E. coli or yeast expression systems. The recombinant protein is used in research to study the biological functions of IL-6 and its role in various diseases. It is also used in the development of diagnostic assays and therapeutic interventions .
Production and Purification Recombinant Canine IL-6 is produced by inserting the gene encoding IL-6 into an expression vector, which is then introduced into a host cell, such as E. coli. The host cells are cultured, and the recombinant protein is expressed and harvested. The protein is then purified using techniques such as affinity chromatography and SDS-PAGE to ensure high purity and activity .
Applications in Research and Medicine Recombinant Canine IL-6 is used in various research applications, including: