IL4R Human
Interleukin 4 Receptor, IL-4 Receptor Subunit Alpha, Interleukin 13 Receptor, IL-4RA, IL4RA, Interleukin-4 Receptor Subunit Alpha, Interleukin-4 Receptor Alpha Chain, IL4R Nirs Variant 1, IL-4R Subunit Alpha, CD124 Antigen, IL-4R-Alpha, CD124, IL4R.
Greater than 95.0% as determined by SDS-PAGE.
Description
IL4R produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (26-232 a.a.) and fused to an 8 aa His Tag at C-terminus containing a total of 215 amino acids and having a molecular mass of 24.7kDa.
IL4R shows multiple bands between 28-40kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
Product Specs
Interleukin 4 (IL4) is a versatile cytokine with a crucial role in the immune system, primarily produced by activated T cells. It binds to the interleukin 4 receptor (IL4R), which also interacts with IL13. This dual binding capacity contributes to the overlapping functions of IL4 and IL13. Signal transduction and activation of transcription (STAT6) is central to IL4's immune regulatory signaling. The genes for IL3, IL4, IL5, IL13, and CSF2 cluster on chromosome 5q, with IL4 positioned close to IL13. Regulation of IL4, IL13, and IL5 is coordinated by multiple long-range regulatory elements spanning over 120 kilobases on the chromosome. Two alternatively spliced transcript variants of the IL4 gene result in distinct isoforms.
Produced in Sf9 Baculovirus cells, IL4R is a single, glycosylated polypeptide chain with a molecular mass of 24.7kDa. It comprises amino acids 26-232 of the IL4R protein and is fused to an 8 amino acid His tag at the C-terminus, resulting in a total of 215 amino acids. On SDS-PAGE under reducing conditions, IL4R exhibits multiple bands between 28-40kDa. Purification is achieved through proprietary chromatographic techniques.
The IL4R protein solution is provided at a concentration of 0.25mg/ml in phosphate-buffered saline (pH 7.4).
SDS-PAGE analysis confirms a purity greater than 95.0%.
Interleukin 4 Receptor, IL-4 Receptor Subunit Alpha, Interleukin 13 Receptor, IL-4RA, IL4RA, Interleukin-4 Receptor Subunit Alpha, Interleukin-4 Receptor Alpha Chain, IL4R Nirs Variant 1, IL-4R Subunit Alpha, CD124 Antigen, IL-4R-Alpha, CD124, IL4R.
MKVLQEPTCV SDYMSISTCE WKMNGPTNCS TELRLLYQLV FLLSEAHTCI PENNGGAGCV CHLLMDDVVS ADNYTLDLWA GQQLLWKGSF KPSEHVKPRA PGNLTVHTNV SDTLLLTWSN PYPPDNYLYN HLTYAVNIWS ENDPADFRIY NVTYLEPSLR IAASTLKSGI SYRARVRAWA
QCYNTTWSEW SPSTKWHNSY REPFEQHLEH HHHHH
Product Science Overview
Interleukin-4 (IL-4) is a pleiotropic cytokine that plays a crucial role in regulating immune responses. It is predominantly secreted by activated CD4+ memory and effector T helper 2 (Tʜ2) cells, basophils, and mast cells . The interleukin-4 receptor (IL-4R) is a critical component in the signaling pathways that mediate the biological effects of IL-4.
The IL-4 receptor is a type I cytokine receptor that exists in two forms: the type I receptor, which is composed of the IL-4R alpha chain (IL-4Rα) and the common gamma chain (γc), and the type II receptor, which consists of IL-4Rα and the interleukin-13 receptor alpha 1 chain (IL-13Rα1) . The binding of IL-4 to its receptor triggers a cascade of signaling events that lead to the activation of various transcription factors, including STAT6, which is essential for the differentiation of naive T cells into Tʜ2 cells .
IL-4 is involved in a wide range of immune responses, including the promotion of B cell proliferation, differentiation, and immunoglobulin class switching . It also plays a pivotal role in the development of allergic inflammation and asthma by promoting the differentiation of Tʜ2 cells and the production of other Tʜ2 cytokines such as interleukin-5 (IL-5) and interleukin-9 (IL-9) .
Recombinant human IL-4 receptor is produced using recombinant DNA technology, which involves the insertion of the IL-4R gene into a suitable expression system, such as Escherichia coli (E. coli), to produce the protein in large quantities . This recombinant protein is used in various research applications, including functional assays, differentiation studies, and cell culture experiments .
Recombinant IL-4 receptor is widely used in immunological research to study the signaling pathways and biological effects of IL-4. It is also used in the development of therapeutic strategies for treating allergic diseases and asthma . The recombinant protein is optimized for use in functional assays, allowing researchers to investigate the mechanisms underlying IL-4-mediated immune responses .
Recent studies have shown that the IL-4 receptor has undergone diversifying selection, particularly at the receptor-binding interfaces . This suggests that the receptor has evolved to avoid pathogen antagonism while maintaining its ability to bind and signal through IL-4. Such evolutionary adaptations highlight the importance of IL-4 and its receptor in host defense mechanisms .
