IL-4 Porcine

Interleukin-4 Porcine Recombinant
Cat. No.
BT17310
Source

Escherichia Coli.

Synonyms

BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.

Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Interleukin-4 Porcine Recombinant produced in E. coli is a non-glycosylated monomer chain containing 110 amino acids and having a molecular mass of 12.7kDa.

IL-4 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Interleukin 4 (IL4) is a cytokine with diverse functions, produced by activated T cells. It binds to the interleukin 4 receptor, which also interacts with IL13, leading to overlapping roles for IL4 and IL13. The signaling pathway activated by IL4 involves STAT6, a protein that regulates gene expression. Genes encoding IL4, IL3, IL5, IL13, and CSF2 are clustered together on chromosome 5q, with IL4 located near IL13. Regulation of IL4, IL13, and IL5 is coordinated by multiple regulatory elements spanning a region greater than 120 kilobases.
Description
Recombinant Porcine Interleukin-4, produced in E. coli, is a non-glycosylated monomeric protein with 110 amino acids. It has a molecular weight of 12.7 kDa. The purification process involves specialized chromatographic techniques.
Physical Appearance
White, lyophilized (freeze-dried) powder that has been sterile filtered.
Formulation
The protein was subjected to sterile filtration (0.2 µm) and lyophilized from a solution containing 10 mM sodium phosphate, pH 7.5.
Solubility
To reconstitute the lyophilized IL-4, it is recommended to dissolve it in sterile 18 MΩ-cm H₂O at a concentration of at least 100 µg/ml. This solution can then be diluted further into other aqueous solutions as needed.
Stability
Lyophilized IL-4 remains stable at room temperature for up to 3 weeks; however, it is recommended to store it desiccated at a temperature below -18°C for long-term storage. After reconstitution, IL-4 should be stored at 4°C for 2-7 days. For extended storage, it should be kept at -18°C. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 95.0% using SDS-PAGE analysis.
Biological Activity
The biological activity, measured by TF-1 cell proliferation, is represented by an ED₅₀ of 0.504 ng/ml. This corresponds to a specific activity of 2.0 x 10⁶ units/mg.
Synonyms

BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.

Source

Escherichia Coli.

Amino Acid Sequence

MHKCDITLQE IIKTLNILTA RKNSCMELPV TDVFAAPENT TEKETFCRAS TVLRHIYRHH TCMKSLLSGL DRNLSSMANM TCSVHEAKKS TLKDFLERLK TIMKEKYSKC.

Product Science Overview

Porcine Interleukin-4 (pIL-4)

Porcine Interleukin-4 (pIL-4) shares similar functions with its human counterpart. It is involved in the immune response regulation in pigs, making it a significant molecule in veterinary immunology. The recombinant form of pIL-4 is produced using genetic engineering techniques, where the pIL-4 gene is inserted into an expression vector and introduced into a host cell to produce the protein in large quantities .

Applications and Importance

Recombinant pIL-4 has been used in various research studies to understand its role in immune responses and its potential as an immunological adjuvant. For instance, a study involving a recombinant Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) expressing pIL-4 demonstrated that the recombinant virus induced higher levels of IL-4 in the blood and increased the percentage of double positive T cells (CD4+CD8+) in vaccinated pigs . This indicates that pIL-4 can enhance the immune response, although it did not significantly improve the protection efficacy of the PRRSV vaccine .

Production of Recombinant pIL-4

The production of recombinant pIL-4 involves several steps:

  1. Gene Cloning: The pIL-4 gene is cloned into an expression vector.
  2. Transformation: The vector is introduced into a suitable host cell, such as E. coli or yeast.
  3. Expression: The host cells are cultured under conditions that promote the expression of the pIL-4 protein.
  4. Purification: The recombinant pIL-4 protein is purified from the host cells using techniques such as affinity chromatography.

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