Escherichia Coli.
BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.
Greater than 95.0% as determined by SDS-PAGE.
Interleukin-4 Canine Recombinant produced in E. coli is a non-glycosylated monomer chain containing 109 amino acids and having a molecular mass of 12.9kDa.
IL-4 is purified by proprietary chromatographic techniques.
BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.
Escherichia Coli.
MHNFNITIKE IIKMLNILTA RNDSCMELTV KDVFTAPKNT SDKEIFCRAA TVLRQIYTHN CSNRYLRGLY RNLSSMANKT CSMNEIKKST LKDFLERLKV IMQKKYYRH.
Interleukin-4 (IL-4) is a cytokine that plays a crucial role in the immune system. It is involved in the regulation of various immune responses, including the activation and differentiation of B cells and T cells. IL-4 is also known as B cell-stimulatory factor-1 (BSF-1) due to its ability to stimulate B cell proliferation .
IL-4 is a glycosylated polypeptide with a molecular weight ranging from approximately 13 kDa to 18 kDa. It contains three intrachain disulfide bridges and adopts a bundled four alpha-helix structure . This structure is essential for its interaction with the IL-4 receptor, which mediates its biological effects.
IL-4 has pleiotropic effects during immune responses. It enhances the secretion and cell surface expression of immunoglobulin E (IgE) and immunoglobulin G1 (IgG1). Additionally, IL-4 regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes .
Recombinant canine IL-4 is produced using recombinant DNA technology. The gene encoding canine IL-4 is cloned and expressed in a suitable host system, such as Escherichia coli (E. coli). The recombinant protein is then purified to achieve high purity levels, typically greater than 95% as determined by SDS-PAGE .
The recombinant canine IL-4 protein consists of 109 amino acids and has a calculated molecular mass of approximately 13 kDa. It is lyophilized from a sterile PBS solution and can be reconstituted for use in various applications .
The biological activity of recombinant canine IL-4 is measured using a cell proliferation assay with TF-1 human erythroleukemic cells. The effective dose (ED50) for this effect is typically in the range of 3-30 ng/mL . IL-4 stimulates the proliferation of activated B cells and T cells, enhancing both secretion and cell surface expression of IgE and IgG1 .
Recombinant canine IL-4 is used in various research applications, including: