IL17B Human, His
Description
IL17B is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Interleukin-17B (IL-17B) is a member of the IL-17 family of cytokines, which are glycoproteins secreted as dimers. These cytokines play a crucial role in inducing local cytokine production and recruiting granulocytes to sites of inflammation. The IL-17 family consists of at least six pro-inflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus .
The human recombinant IL-17B is produced in Escherichia coli and is a single, non-glycosylated polypeptide chain containing 185 amino acids (21-180) with a molecular mass of approximately 20 kDa. This recombinant protein is fused to a 25 amino acid His-tag at the N-terminus, which facilitates its purification through proprietary chromatographic techniques .
IL-17B binds to the IL-17B receptor but not to the IL-17 receptor. It is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17B is involved in various inflammatory processes and has been associated with diseases such as spondyloarthropathy and neuronitis. It plays a role in several signaling pathways, including the IL-17 signaling pathways and the STAT3 pathway .
The human recombinant IL-17B with a His-tag is primarily used for laboratory research purposes. It is utilized in studies related to cytokine activity, inflammatory responses, and disease mechanisms. The His-tag allows for easy purification and detection of the protein in various experimental setups .
The IL-17B solution is typically formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.4 M urea, and 10% glycerol. For short-term storage, it can be kept at 4°C if used within 2-4 weeks. For long-term storage, it is recommended to store the protein at -20°C with the addition of a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) to prevent freeze-thaw cycles .
