IL17B Human, Sf9

Interleukin-17B (IL-17B) is a member of the IL-17 family of cytokines, which are glycoproteins secreted as dimers. These cytokines play a crucial role in inducing local cytokine production and recruiting granulocytes to sites of inflammation . The IL-17 family consists of at least six pro-inflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus .

IL-17B Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 169 amino acids (21-180a.a.) and has a molecular mass of 19.2 kDa . The molecular size on SDS-PAGE appears at approximately 18-28 kDa . This recombinant protein is expressed with a 6 amino acid His-tag at the C-terminus and is purified using proprietary chromatographic techniques .

IL-17B binds to the IL-17B receptor but not to the IL-17 receptor. It is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells . IL-17B is involved in various biological pathways, including the IL-6, IL-17 signaling pathways, and the STAT3 pathway . Diseases associated with IL-17B include spondyloarthropathy and neuronitis .

The IL-17B protein solution is a sterile filtered colorless solution formulated in phosphate-buffered saline (pH 7.4) with 10% glycerol . The protein has a purity greater than 90.0% as determined by SDS-PAGE . The amino acid sequence of IL-17B is as follows:

ADP QPRSPKS KRKGQGRPGP LAPGPHQVPL DLVSRMKPYA RMEEYERNIE EMVAQLRNSS ELAQRKCEVN LQLWMSNKRS LSPWGYSINH DPSRIPVDLP EARCLCLGCV NPFTMQEDRS MVSVPVFSQV PVRRRLCPPP PRTGPCRQRA VMETIAVGCT CIF HHHHHH

IL-17B should be stored at 4°C if the entire vial will be used within 2-4 weeks. For longer periods, it should be stored frozen at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA) and avoid multiple freeze-thaw cycles .

IL-17B is used primarily for laboratory research purposes. It is not intended for use as drugs, agricultural or pesticidal products, food additives, or household chemicals .