IL 17B Human

Interleukin 17B Human Recombinant
Cat. No.
BT31021
Source
Escherichia Coli.
Synonyms
Interleukin-17B, IL-17B, Cytokine Zcyto7, Interleukin-20, Neuronal interleukin-17-related factor, IL20, NIRF, ZCYTO7.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

Interleukin-17B Human Recombinant produced in E.Coli is a homodimeric, non-disulfide-linked polypeptide chain containing a total of 322 amino acids (2 chains of 161aa) and having a molecular mass of 36.5kDa. The IL-17B is purified by proprietary chromatographic techniques.

Product Specs

Introduction
The IL-17 family consists of several pro-inflammatory cytokines that play a role in immune responses and inflammation. IL-17B, a member of this family, is involved in various inflammatory processes and immune regulation. It interacts with the IL-17B receptor, influencing the production of cytokines and the recruitment of immune cells.
Description
Recombinant human Interleukin-17B, a protein crucial for immune system function, is produced in E. coli. This process ensures a highly pure and active form of the protein, ideal for research and laboratory applications. The protein exists as a dimer, with each chain comprising 161 amino acids, resulting in a total molecular weight of 36.5 kDa. Its purity is rigorously maintained through specialized chromatographic techniques.
Physical Appearance
White, lyophilized powder, sterilized by filtration.
Formulation
To ensure stability, the Interleukin-17B protein undergoes lyophilization, a freeze-drying process. The protein is formulated in a phosphate-buffered saline solution (PBS) with a pH of 7.4, containing 0.05% Tween-20 and 3% Trehalose.
Solubility
For reconstitution, sterile 18 megaohm-centimeter (MΩ·cm) H₂O is recommended, with a minimum concentration of 100 micrograms per milliliter (µg/ml). Further dilutions can be made using other aqueous solutions as needed.
Stability
Lyophilized Interleukin-17B is stable at room temperature for up to 3 weeks; however, for extended storage, it is recommended to store it desiccated below -18 degrees Celsius. After reconstitution, IL-17B should be stored at 4 degrees Celsius for 2-7 days. For long-term storage, freezing below -18 degrees Celsius is recommended. It is advisable to add a carrier protein (0.1% HSA or BSA) for enhanced stability during long-term storage. Avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of the Interleukin-17B protein exceeds 95%, as determined by rigorous analysis using Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) and SDS-PAGE.
Biological Activity
The biological activity of Interleukin-17B is assessed by its ability to induce IL-8 secretion in human HepG2 cells. The ED50, which represents the concentration required for half-maximal response, is less than 1.0 µg/ml. This corresponds to a specific activity greater than 1000 international units per milligram (IU/mg).
Synonyms
Interleukin-17B, IL-17B, Cytokine Zcyto7, Interleukin-20, Neuronal interleukin-17-related factor, IL20, NIRF, ZCYTO7.
Source
Escherichia Coli.
Amino Acid Sequence
MQPRSPKSKR KGQGRPGPLA PGPHQVPLDL VSRMKPYARM EEYERNIEEM VAQLRNSSEL AQRKCEVNLQ LWMSNKRSLS PWGYSINHDP SRIPVDLPEA RCLCLGCVNP FTMQEDRSMV SVPVFSQVPV RRRLCPPPPR TGPCRQRAVM ETIAVGCTCI F.

Product Science Overview

Introduction

Interleukin 17B (IL-17B) is a member of the IL-17 cytokine family, which plays a crucial role in immune responses and inflammatory processes. The IL-17 family consists of six cytokines: IL-17A, IL-17B, IL-17C, IL-17D, IL-17E, and IL-17F. These cytokines are known for their involvement in host defense mechanisms and various inflammatory diseases .

Discovery and Cloning

Human IL-17B was first cloned in 2000 through homology-based screening of an expressed sequence tag database, followed by amplification from a fetal tissue cDNA library . The IL-17B protein shares 88% homology with its murine ortholog but only 29% homology with human IL-17A .

Structure and Properties

Recombinant Human IL-17B is typically produced in an E. coli expression system and is a non-disulfide-linked homodimer consisting of two 161 amino acid polypeptide chains. The molecular weight of this recombinant protein is approximately 36.6 kDa . It is highly purified, with a purity of ≥ 98% as determined by SDS-PAGE gel and HPLC analyses . The endotoxin concentration is less than 1 EU/µg .

Biological Activity

IL-17B is known to stimulate the release of pro-inflammatory cytokines such as TNF alpha (TNF) and IL1 beta (IL1B) from monocytic cell lines . It also induces IL-8 secretion in human PBMCs (Peripheral Blood Mononuclear Cells) and HepG2 human hepatocellular carcinoma cells . The activity of IL-17B is measured by its ability to induce IL-8 secretion, with an effective dose (ED50) ranging from 0.4 to 2 µg/mL .

Localization and Function

Immunohistochemical analysis has shown that IL-17B is primarily localized to neuronal cell bodies . It plays a significant role in inflammation and bone growth . The IL-17B/IL-17RB pathway has also been implicated in cancer, highlighting its importance in various physiological and pathological processes .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

IL17A Human, Sf9
Interleukin 17A Human Recombinant, Sf9
Cat. No.
BT9227
Source
Sf9, Baculovirus cells.
IL17B Human, His
Interleukin-17B Human Recombinant, His Tag
Cat. No.
BT9401
Source
Escherichia Coli.
IL17D Human
Interleukin-17D Human Recombinant
Cat. No.
BT9563
Source
Escherichia Coli.
IL17RB Human
Interleukin-17 Receptor Beta Human Recombinant
Cat. No.
BT10277
Source
<p>Sf9, Baculovirus cells.</p>
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.