IL-6 PAT1F10AT Antibody

Interleukin-6 (IL-6) is a multifunctional cytokine that plays a crucial role in immune responses, inflammation, and hematopoiesis. The IL-6 Clone PAT1F10AT is a monoclonal antibody derived from mouse and specifically targets human IL-6. This antibody is widely used in immunological research to study the biological functions and mechanisms of IL-6.

The IL-6 Clone PAT1F10AT antibody is produced by hybridizing mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human IL-6 protein. The antibody belongs to the IgG1 subclass and contains a kappa light chain . It is purified from mouse ascitic fluids using protein-A affinity chromatography, ensuring high purity and specificity .

IL-6 is involved in a wide range of biological processes:

• Immune Response: IL-6 plays a pivotal role in the final differentiation of B-cells into immunoglobulin-secreting cells .
• Inflammation: It is a key mediator of the acute phase response and fever .
• Hematopoiesis: IL-6 regulates the production of blood cells in the bone marrow .
• Nerve Cell Differentiation: It induces the differentiation of nerve cells .

The IL-6 Clone PAT1F10AT antibody is used in various applications, including:

• Enzyme-Linked Immunosorbent Assay (ELISA): To detect and quantify IL-6 levels in biological samples.
• Western Blot Analysis: To confirm the presence of IL-6 in protein extracts .
• Functional Studies: To investigate the role of IL-6 in different biological processes .

The antibody is formulated in a phosphate-buffered saline (PBS) solution with 10% glycerol and 0.02% sodium azide. It should be stored at -20°C for long-term storage and at 4°C for short-term use. The antibody is stable for up to 12 months at -20°C and for 1 month at 4°C .