Introduction
This gene encodes a cytokine that plays a crucial role in the growth and differentiation of B cells and eosinophils. It acts as a key regulator for eosinopoiesis, which includes the maturation and activation of eosinophils. Elevated levels of this cytokine have been linked to asthma and hypereosinophilic syndromes. Its receptor is a heterodimer, sharing the beta subunit with receptors for interleukin 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). Located on chromosome 5, this gene clusters with genes for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, and their regulation is coordinated by long-range regulatory elements spanning 120 kilobases on chromosome 5q31.
Description
Recombinant Rat Interleukin-5, produced in E. coli, is a dimeric, non-glycosylated polypeptide consisting of 113 amino acids. It has a molecular weight of 13074 Daltons and is purified using proprietary chromatographic techniques.
Physical Appearance
White, sterile-filtered powder, freeze-dried.
Formulation
Lyophilized from a 1 mg/ml solution in water, without any additives.
Solubility
For reconstitution, it is recommended to dissolve the lyophilized Interleukin-5 Rat in sterile 18MΩ-cm H2O at a concentration not less than 100 µg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized Interleukin-5 Rat remains stable at room temperature for 3 weeks. However, it is recommended to store it desiccated below -18°C. After reconstitution, store Interleukin-5 Rat at 4°C for 2-7 days. For long-term storage, store below -18°C. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 95.0%, as determined by: (a) RP-HPLC analysis and (b) SDS-PAGE analysis.
Biological Activity
The ED50 ranges from 0.3 to 1.0 ng/ml, as measured by the dose-dependent stimulation of BCL-1 cell proliferation.
Synonyms
EDF, BCDFII, TRF, T-cell replacing factor, Eosinophil differentiation factor, B cell differentiation factor I, IL-5.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Ile-Pro-Met.
