IL 5 Human

Interleukin-5 Human Recombinant
Cat. No.
BT5674
Source
Escherichia Coli.
Synonyms
EDF, BCDFII, TRF, T-cell replacing factor, Eosinophil differentiation factor, B cell differentiation factor I, IL-5.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Interleukin-5 Human Recombinant produced in E.Coli is a dimeric, non-glycosylated polypeptide chain containing two 113 amino acids chains, and having a molecular mass of 26522.84 Dalton. The IL-5 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Interleukin-5 (IL-5) is a cytokine crucial for the growth and differentiation of B cells and eosinophils. It plays a significant role in eosinopoiesis, regulating eosinophil maturation and activation. Elevated IL-5 levels are implicated in conditions like asthma and hypereosinophilic syndromes. The IL-5 receptor is a heterodimer, sharing its beta subunit with the receptors for IL-3 and GM-CSF. The genes for IL-5, IL-4, IL-13, and CSF2 form a cluster on chromosome 5, regulated by long-range elements spanning 120 kilobases on chromosome 5q31.
Description
Recombinant Human Interleukin-5, produced in E. coli, is a non-glycosylated polypeptide comprising two identical chains, each containing 113 amino acids. This dimeric protein has a molecular mass of 26.5 kDa. The purification process involves proprietary chromatographic techniques.
Physical Appearance
White, sterile-filtered powder obtained through lyophilization (freeze-drying).
Formulation
The product is lyophilized from a 1 mg/ml aqueous solution without any additives.
Solubility
Reconstitute the lyophilized Interleukin-5 in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. Further dilutions can be made in other aqueous solutions.
Stability
Lyophilized Interleukin-5 remains stable at room temperature for up to 3 weeks. However, for long-term storage, it should be kept desiccated at a temperature below -18°C. After reconstitution, store IL-5 at 4°C for a maximum of 7 days. For extended storage, store below -18°C. Avoid repeated freeze-thaw cycles.
Purity
Exceeds 98.0% purity, as determined by: (a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis and (b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
Biological Activity
The ED50, determined by the dose-dependent stimulation of TF-1 cell proliferation, is less than 0.15 ng/ml. This corresponds to a specific activity greater than 6 x 106 IU/mg.
Synonyms
EDF, BCDFII, TRF, T-cell replacing factor, Eosinophil differentiation factor, B cell differentiation factor I, IL-5.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ile-Pro-Thr-Glu.

Product Science Overview

Structure and Function

IL-5 is a disulfide-linked homodimer with a molecular mass of approximately 13 kDa per monomer . It is primarily produced by T helper cells, mast cells, and eosinophils . The cytokine acts as a growth and differentiation factor for both B cells and eosinophils . It is a key regulator of eosinopoiesis, eosinophil maturation, and activation .

Biological Activity

IL-5’s primary function is to stimulate the proliferation and activation of eosinophils . It also promotes the secretion of immunoglobulins by B cells and the generation of cytotoxic T cells . Through the activation of eosinophils, IL-5 mediates several allergic diseases, including asthma and hypereosinophilic syndromes .

Recombinant Human IL-5

Recombinant human IL-5 is a form of IL-5 that is produced using recombinant DNA technology. This involves inserting the gene encoding IL-5 into a host cell, such as E. coli or insect cells, which then produces the protein . Recombinant IL-5 is used in various research applications, including cell culture, differentiation studies, and functional assays .

Applications

Human recombinant IL-5 is widely used in scientific research to study:

  • The cultivation and differentiation of eosinophils .
  • Allergic diseases and their mechanisms .
  • Parasitic infections and tumor development .
Quality and Purity

Research-grade recombinant IL-5 is typically sterile-filtered and lyophilized, with endotoxin levels below 0.1 ng/μg and purities greater than 95% . The biological activity of recombinant IL-5 is tested in appropriate bioassays to ensure its efficacy .

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