Introduction
Interleukin-5 (IL-5) is a cytokine crucial for the growth, differentiation, and activation of eosinophils, which are involved in immune responses, particularly against parasites and in allergic reactions. IL-5's role in eosinopoiesis makes it a key player in conditions like asthma and hypereosinophilic syndromes. It exerts its effects by binding to a specific receptor (a heterodimer) on target cells, sharing a subunit with the receptors for IL-3 and CSF2. The gene encoding IL-5 is located within a cytokine gene cluster on chromosome 5, alongside genes for IL-4, IL-13, and CSF2. Notably, these cytokines are co-regulated by long-range regulatory elements spanning a significant portion of chromosome 5q31.
Description
IL-5 Rhesus Macaque Recombinant is a manufactured protein similar to the naturally occurring IL-5 found in Rhesus Macaque monkeys. It is produced using E. coli bacteria as a host for protein expression. This recombinant protein is a homodimer, meaning it consists of two identical polypeptide chains linked by disulfide bonds. Each chain contains 115 amino acids, and the protein lacks glycosylation. With a total molecular weight of 26.1 kDa, the protein is purified using specialized chromatography techniques to ensure high purity.
Physical Appearance
The product is provided as a white powder, which has been sterilized by filtration and then freeze-dried.
Formulation
The protein is freeze-dried from a solution containing phosphate-buffered saline (PBS) at a pH of 7.4, with trehalose added as a stabilizing agent. The solution is filtered through a 0.2 micrometer filter before freeze-drying to ensure sterility.
Solubility
To reconstitute the lyophilized protein, it is recommended to dissolve it in sterile, high-purity water (18 megaohm-cm resistivity) at a concentration of at least 100 micrograms per milliliter. The resulting solution can then be further diluted in other aqueous solutions as needed.
Stability
While the lyophilized IL-5 Rhesus Macaque remains stable at room temperature for up to three weeks, it is recommended to store it desiccated at -18°C or lower for long-term storage. After reconstitution, the solution should be stored at 4°C and used within 2 to 7 days. For extended storage, it can be stored at -18°C or lower, avoiding repeated freeze-thaw cycles to preserve protein integrity.
Purity
The purity of the IL-5 Rhesus Macaque Recombinant protein is determined to be greater than 98% using High-Performance Liquid Chromatography (HPLC) and SDS-PAGE analysis.
Biological Activity
The biological activity of the protein is assessed through its ability to stimulate cell proliferation in a specific cell line (human TF-1 cells). The ED50 value, which represents the effective concentration required to achieve 50% of the maximum response, is determined to be less than 5 nanograms per milliliter. This corresponds to a specific activity greater than 2.0 x 10^5 international units per milligram, indicating the potency of the protein in stimulating cell proliferation.
Synonyms
Interleukin-5, IL-5, Eosinophil differentiation factor, T-cell replacing factor, TRF, IL5.
Amino Acid Sequence
IPTEIPASAL VKETLALLST HRTLLIANET LRIPVPVHKN HQLCTEEIFQ GIGTLESQTV QGGTVERLFK NLSLIKKYIG GQKKKCGEER RRVNQFLDYL QEFLGVMNTE WIIES.
