IGF1 Human
Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA MGF.
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
IGF1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.6kDa.
IGF-1 is purified by proprietary chromatographic techniques.
Product Specs
The somatomedins, also known as insulin-like growth factors (IGFs), are a family of peptides that play a crucial role in the growth and development of mammals. IGF1 is a key mediator of growth hormone's (GH) growth-promoting effects. Initial research revealed that GH did not directly stimulate sulfate incorporation into cartilage. Instead, it acted through a serum factor initially called 'sulfation factor' and later renamed 'somatomedin.' Three primary somatomedins have been identified: somatomedin C (IGF1), somatomedin A (IGF2), and somatomedin B.
Recombinant Human IGF1 produced in E. coli is a single, non-glycosylated polypeptide chain consisting of 70 amino acids. It has a molecular weight of 7.6 kDa. The purification of IGF-1 is achieved using proprietary chromatographic methods.
The protein was freeze-dried from a concentrated solution in phosphate-buffered saline (PBS) at a pH of 7.0.
Lyophilized IGF1 remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it in a desiccated state at a temperature below -18°C. After reconstitution, IGF1 should be stored at 4°C for 2 to 7 days. For extended storage, it is advisable to add a carrier protein such as HSA or BSA at a concentration of 0.1%. Avoid repeated freeze-thaw cycles.
The purity of IGF-1 is greater than 97.0%, as determined by the following methods:
(a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis.
(b) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
The biological activity, assessed using a cell proliferation assay with serum-free human MCF-7 cells, is less than 2 ng/ml. This corresponds to a specific activity greater than 5.0 x 105 IU/mg.
Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA MGF.
Product Science Overview
IGF-1 is a single-chain polypeptide consisting of 70 amino acids with a molecular weight of approximately 7.6 kDa . It shares a high degree of homology with proinsulin, which is reflected in its ability to bind to insulin receptors, albeit with lower affinity . The primary function of IGF-1 is to mediate the effects of growth hormone, promoting growth and development in tissues such as muscle, bone, and cartilage .
Recombinant human IGF-1 is produced using recombinant DNA technology, typically in Escherichia coli (E. coli) expression systems . This method involves inserting the human IGF-1 gene into the bacterial DNA, allowing the bacteria to produce the protein. The recombinant protein is then purified and used for various research and therapeutic applications.
- Research: Recombinant IGF-1 is widely used in scientific research to study its effects on cell growth, differentiation, and survival. It is particularly useful in cancer research, where it is used to investigate the role of IGF-1 in tumor growth and progression .
- Therapeutics: IGF-1 has potential therapeutic applications in treating growth disorders, such as growth hormone insensitivity syndrome (GHIS) and Laron syndrome. It is also being explored for its potential in treating neurodegenerative diseases like Alzheimer’s disease, as low levels of IGF-1 have been linked to the condition .
Recombinant human IGF-1 is typically lyophilized and stored at -20°C to -80°C to maintain its stability . It is essential to avoid repeated freeze-thaw cycles to prevent degradation of the protein. Once reconstituted, the protein should be stored under sterile conditions at 2°C to 8°C for short-term use or at -20°C to -80°C for long-term storage .
