IGF1 N15 Human
Insulin Like Growth Factor-1 N15 Labeled Human Recombinant
Cat. No.
BT18271
Source
Escherichia Coli.
Synonyms
Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
IGF1 N15 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 70 amino acids and having a molecular mass of 7.74kDa. The N15 is stable isotope labeled.
The IGF1 N15 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Insulin-like growth factors (IGFs), including somatomedins, are peptides crucial for mammalian growth and development. IGF1, in particular, is a key mediator of growth hormone's growth-promoting effects. Growth hormone relies on IGF1, originally known as 'sulfation factor' or 'somatomedin,' to stimulate sulfate incorporation into cartilage. This family of somatomedins includes IGF1, IGF2 (MIM 147470), and somatomedin B (MIM 193190).
Description
Recombinant human IGF1 N15, produced in E.coli, is a single, non-glycosylated polypeptide chain consisting of 70 amino acids. With a molecular mass of 7.74kDa, it features stable isotope labeling with N15. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
White lyophilized powder, sterile filtered.
Formulation
The lyophilization of IGF1 N15 protein was carried out from a 0.2µm filtered concentrated solution in phosphate-buffered saline (PBS) at a pH of 7.2.
Solubility
For reconstitution of lyophilized IGF1 N15, sterile 18M-cm H2O at a concentration of at least 100µg/ml is recommended. This solution can be further diluted as needed in other aqueous solutions.
Stability
Lyophilized IGF1 N15 remains stable at room temperature for up to 3 weeks. However, for extended storage, it is recommended to store it desiccated at a temperature below -18°C. After reconstitution, IGF1 N15 should be stored at 4°C for 2-7 days. For long-term storage, storing below -18°C is advised. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. It is essential to avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 97.0%, as determined by Reverse Phase High-Performance Liquid Chromatography (RP-HPLC) and Sodium Dodecyl-Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) analyses.
Biological Activity
The half-maximal effective concentration (ED50) is less than 2ng/ml, as determined by a cell proliferation assay utilizing serum-free human MCF-7 cells. This corresponds to a specific activity greater than 5.0 x 105 IU/mg.
Synonyms
Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF.
Source
Escherichia Coli.
Amino Acid Sequence
GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA.
Product Science Overview
Structure and Function
IGF-1 is a globular protein containing 70 amino acids and three intra-molecular disulfide bonds . It is generated through the proteolytic processing of inactive precursor proteins . The expression of IGF-1 is regulated by growth hormone, while IGF-2 expression is influenced by placental lactogen . Both IGF-1 and IGF-2 signal through the tyrosine kinase type I receptor (IGFIR) .
N15 Labeling
Biological Properties
IGF-1 has several biological properties, including:
- Cell Proliferation: IGF-1 stimulates the proliferation of a wide range of cell types in muscle, bone, and cartilage tissue .
- Cell Differentiation: It induces cellular differentiation, promoting the development of specialized cell types .
- Cell Migration: IGF-1 enhances the migration of cells, which is crucial for tissue repair and regeneration .
- Cell Survival: It promotes cell survival by inhibiting apoptosis (programmed cell death) .
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