IgA 1+2 antibody
IgA 1&2, Mouse Anti-Human
Cat. No.
BT20600
Source
Synonyms
Appearance
Purity
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
Product Specs
Formulation
Provided as a 1 milligram per milliliter solution in phosphate-buffered saline upon reconstitution.
Shipping Conditions
The antibody is shipped in a lyophilized form at ambient temperature.
Storage Procedures
For long-term storage in lyophilized form, keep at 4 degrees Celsius in a dry environment. After reconstitution, aliquot and store at -20 degrees Celsius if not intended for use within one month. Avoid repeated freeze-thaw cycles.
Solubility
To obtain a 1 milligram per milliliter solution, reconstitute with H2O. Mix gently, ensuring the sides of the vial are washed, and allow 30-60 seconds for complete dissolution before use.
Titer
Direct ELISA analysis indicates that a 1:20,000 dilution will result in an optical density greater than 1.0 when using alkaline phosphatase conjugated rabbit anti-mouse immunoglobulin G (Jackson Laboratories).
Note
This antibody exhibits binding affinity for both human immunoglobulin A subtype 1 and human immunoglobulin A subtype 2.
Purification Method
Ion exchange column.
Type
Mouse Anti Human Monoclonal.
Immunogen
Purified hIgA.
Ig Subclass
Mouse IgG1.
Product Science Overview
IgA1 and IgA2
- IgA1: This subclass is more prevalent in serum and is known for its role in immune defense mechanisms. It has a longer hinge region, which makes it more susceptible to proteolytic cleavage by bacterial proteases.
- IgA2: This subclass is more resistant to bacterial proteases due to its shorter hinge region. It is predominantly found in mucosal secretions and plays a vital role in protecting mucosal surfaces from infection.
Mouse Anti-Human IgA1 & IgA2 Antibodies
Mouse anti-human IgA1 & IgA2 antibodies are monoclonal antibodies specifically designed to react with human IgA1 and IgA2. These antibodies are used in various immunological assays to detect and quantify IgA levels in human samples.
- Source: These antibodies are typically derived from highly purified monoclonal IgA isolated from human serum .
- Specificity: The reactivity of the antiserum is restricted to determinants on the CH2 domain of IgA. It reacts with both subclasses of IgA as tested in various assays such as haemagglutination, enzyme immunoassays, immunoblotting, and immunoprecipitation .
- Applications: These antibodies are used in techniques like radioimmunoassay, ELISA, indirect immunoperoxidase, immunofluorescence staining, haemagglutination, and immunoblotting to identify the presence of IgA in human serum, other body fluids, cell, and tissue substrates .
Storage and Handling
- Storage: The lyophilized product is shipped at ambient temperature and may be stored at +4°C. For prolonged storage, it should be kept at or below -20°C. Diluted solutions should be stored at +4°C and not refrozen .
- Handling: It is essential to avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, it should be removed by centrifugation, which will not affect the performance of the product .
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