IgG-Fc antibody

Immunoglobulin G (IgG) is a type of antibody that plays a crucial role in the immune response by identifying and neutralizing pathogens such as bacteria and viruses. The Fc region (Fragment crystallizable region) of IgG is responsible for binding to cell surface receptors and mediating various immune functions. Mouse Anti-Human IgG-Fc antibodies are monoclonal antibodies derived from mice that specifically target the Fc region of human IgG. These antibodies are widely used in research and diagnostic applications.

IgG antibodies are composed of two heavy chains and two light chains, forming a Y-shaped structure. The Fc region is located at the base of the Y and is responsible for binding to Fc receptors on immune cells. This binding triggers various immune responses, including phagocytosis, antibody-dependent cellular cytotoxicity (ADCC), and complement activation .

Mouse Anti-Human IgG-Fc antibodies are produced by immunizing mice with the Fc region of human IgG. The spleen cells from these immunized mice are then fused with myeloma cells to create hybridoma cells. These hybridoma cells are capable of producing large quantities of monoclonal antibodies that specifically target the Fc region of human IgG .

1. Research: These antibodies are commonly used in immunoassays, such as ELISA (Enzyme-Linked Immunosorbent Assay), to detect and quantify human IgG levels in various samples. They are also used in Western blotting, immunoprecipitation, and flow cytometry to study protein-protein interactions and immune responses .
2. Diagnostics: In clinical diagnostics, Mouse Anti-Human IgG-Fc antibodies are used to detect the presence of human IgG in patient samples. This can help diagnose various conditions, including autoimmune diseases, infections, and immunodeficiencies .
3. Therapeutics: These antibodies are also being explored for therapeutic applications, such as targeting specific immune cells in autoimmune diseases and cancer .

• Advantages: Mouse Anti-Human IgG-Fc antibodies offer high specificity and affinity for the Fc region of human IgG. They are relatively easy to produce and can be generated in large quantities. Additionally, they are versatile and can be used in a wide range of applications .
• Limitations: One limitation is the potential for immunogenicity when used in human patients, as the mouse-derived antibodies can be recognized as foreign by the human immune system. This can lead to the production of anti-mouse antibodies, which may reduce the efficacy of the treatment .