IgM Antibody
Description
Product Specs
IgM antibody was purified from mouse ascitic fluids by Protein-A chromatography.
Product Science Overview
IgM is a large pentameric molecule composed of five monomeric units, each consisting of two light chains and two heavy chains. The heavy chains in IgM contain four constant immunoglobulin domains, unlike the three found in IgG antibodies . These monomers are linked together by a small polypeptide called the J-chain, forming a structure that allows IgM to effectively bind to antigens and activate the immune response .
Mouse anti-human IgM antibodies are monoclonal or polyclonal antibodies developed in mice that specifically target human IgM. These antibodies are widely used in various research and diagnostic applications, including:
- Flow Cytometry: Used to analyze the expression of IgM on the surface of cells.
- Immunohistochemistry (IHC): Used to detect IgM in tissue samples.
- Western Blotting: Used to identify IgM proteins in a sample.
- Enzyme-Linked Immunosorbent Assay (ELISA): Used to quantify IgM levels in biological samples .
Mouse anti-human IgM antibodies are essential tools in immunology and biomedical research. They help in understanding the role of IgM in the immune response, diagnosing diseases, and developing therapeutic strategies. These antibodies are also used in the study of autoimmune diseases, infections, and immunodeficiencies.
Several companies offer mouse anti-human IgM antibodies, each with different conjugates and specificities to suit various experimental needs. For example, Invitrogen and BD Biosciences provide a range of monoclonal and polyclonal antibodies that have been validated for use in multiple applications .
